免疫比浊法测定抗环瓜氨酸肽抗体的临床应用

目的 评价免疫比浊法检测抗环瓜氨酸肽抗体(CCP)在类风湿关节炎(RA)诊断中的临床价值。方法 收集195例RA、105例骨关节炎、52例骨质疏松、42例结缔组织、40例系统性红斑狼疮、305例其他疾病患者,以及90例健康对照者的血清标本,分别使用免疫比浊法(Latex)、化学发光微粒子免疫法(CMIA)、酶联免疫吸附试验法(ELISA)检测CCP并比较结果。结果 Latex法检测RA阳性141例(72.30%),关节炎阳性54例(51.42%),骨质疏松阳性15例(28.84%),结缔组织阳性15例(35.71%),系统性红斑狼疮阳性18例(45.0%),其他疾病阳性11例(3.60%),90例健康对照者均为阴性。批内变异系数为1.9%~2.0%,总变异系数为2.0%~2.1%,Latex法试剂线性范围为0.8~100U/mL。Latex法、CMIA法、ELISA法检测CCP的敏感性和特异性差异无统计学意义(P0.05)。结论 Latex法检测CCP的敏感性和特异性好,线性范围宽,可用于临床。

The clinical application to detect the anti cyclic citrullinated peptide antibody by immune Turbidimetry

Objective To evaluate the clinical application value of anti cyclic citrullinated peptide antibody(CCP) by immune tur‐bidity method for the the diagnosis of rheumatoid arthritis (RA) .Methods The serum of 195 cases of RA patients ,105 cases of os‐teoarthritis ,52 cases of osteoporosis ,42 cases of connective tissue ,40 patients with systemic lupus erythematosus ,305 cases of pa‐tients with other diseases and 90 cases of healthy controls were collected ,the CCP were detected by latex CMIA and ELISA . Results The CCP positive were 141 cases in RA(72 .30% ) ,54 cases(51 .42% ) in arthritis ,15 cases in osteoporosis (28 .84% ) ,15 cases in connective tissue(35 .71% ) ,18 cases (45 .0% ) in systemic lupus erythematosus (SLE) ,11 cases (3 .60% ) in other disea‐ses .There were negative in 90 cases of healthy controls .The variation coefficient was 1 .9% -2 .0% ,and the total coefficient of var‐iation was 2 .0% -2 .1% .The linear range of Latex method was divided into 0 .8-100 U/mL .There were no diffrence in the sensi‐tivity and specificity of detection to CCP by CMIA ,ELISA and Latex .Conclusion The sensitivity and specificity of detection to CCP by Latex was good ,and there was wide linear range .