首页 | 本学科首页   官方微博 | 高级检索  
检索        

尿血红蛋白对苄索氯铵法定量测定尿蛋白的干扰评价
引用本文:孙宁娜,李洪春.尿血红蛋白对苄索氯铵法定量测定尿蛋白的干扰评价[J].国际检验医学杂志,2016(20):2836-2837.
作者姓名:孙宁娜  李洪春
作者单位:徐州医学院附属医院检验科,江苏徐州,221002
摘    要:目的评价尿血红蛋白对苄索氯铵法定量测定尿蛋白的影响。方法参照美国临床和实验室标准化协会(CLSI)的EP7-A2方案,配制不同浓度血红蛋白的尿液标本做剂量-效应试验,同时收集50例不同隐血程度的尿液标本做临床标本的偏倚试验;尿蛋白定量在DPP罗氏生化工作站采用苄索氯铵法检测,与磺基水杨酸法进行比较。结果剂量-效应试验显示,当尿血红蛋白浓度达0.2g/L时即对苄索氯铵法测定尿蛋白产生显著性干扰(P0.05);临床标本的偏倚试验显示,不同程度的隐血对苄索氯铵法测定尿蛋白产生明显的正干扰。结论重视尿隐血阳性和/或含有红细胞的尿液标本,在采用苄索氯铵法测定尿蛋白时应评价其准确性,必要时使用磺基水杨酸法进行确认。

关 键 词:血红蛋白  苄索氯铵法  尿蛋白定量  干扰

Evaluation of the interference of hemoglobin to the determination of protein in urine by benzethonium chloride
Abstract:Objective To evaluate the interference of occult blood in urine (urinary hemoglobin) and to determine the protein in urine by benzethonium chloride .Methods By reference to EP7‐A2 of CLSI ,urine containing different concentrations of hemoglobin were preparated to produce the "dose‐effect curve",while 50 cases of clinical urine with varying degrees of occult blood were col‐lected for "clinical sample bias test".Urinary protein was quantified by benzyl chloride in DPP roche comparing with sulfosalicylic acid method .Results When the urinary hemoglobin was 0 .2 g/L or higher ,the determined result of urinary protein of urinary pro‐tein would be significantly interfered by benzethonium chloride method .The "clinical sample bias test"showed that clinical urine with different degrees of occult blood caused positive interference to the determination of protein by benzethonium chloride .Conclu‐sion Urine samples with occult blood and /or containing red blood cells should be emphasized and evaluate the accuracy when the urinary protein is quantified by benzyl chloride .If necessary ,it should be confirmed by sulfosalicylic acid .
Keywords:hemoglobin  benzethonium chloride method  urinary protein  interference
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号