血清抗微生物抗体检测在炎性肠病临床诊断中的应用

目的分析血清抗微生物抗体检测在炎性肠病临床诊断中的应用。方法将炎性肠病76例分为克罗恩病(CD)组与溃疡性结肠炎(UC)组。2组空腹采集静脉血2mL,经离心处理后收集血清,采用间接免疫荧光法检测免疫球蛋白G(IgG)型与免疫球蛋白A(IgA)型抗酿酒酵母菌抗体(ASCA)、抗胰腺腺泡抗体(PAB)及抗小肠杯状细胞抗体(GAB),同时检测抗中性粒细胞细胞质抗体(pANCA)。结果 UC组ASCA-IgG和IgA的一种以上亚型阳性率低于CD组,其GAB、pANCA阳性率高于CD组(P0.05);2组PAB均未有阳性;CD组ASCA阳性特异性、敏感性为96.53%、18.75%;UC组pANCA阳性特异性、敏感性为96.37%、53.33%。结论血清抗微生物抗体检测可为炎性肠病临床诊断提供依据。

Application of serum antimicrobial antibody detection in clinical diagnosis of inflammatory bowel disease in clinical diagnosis

Objective To analyze the application of antimicrobial antibody detection in the clinical diagnosis of inflammatory bowel disease .Methods Seventy‐six cases of inflammatory bowel disease were divided into the Crohn′s disease(CD)group and ul‐cerative colitis(UC)group .2 mL of fasting blood were collected in the two group and centrifuged for collecting serum .The levels of IgG ,IgA ,ASCA ,PAB and GAB were detected by adopting the indirect immunofluorescence method ,meanwhile pANCA was detec‐ted .Results The positive rate of more than 1 subtype of ASCA IgG and IgA in the UC group was lower than that in the CD group , its GAB ,pANCA positive rates were higher than those of the CD group (P<0 .05);the two groups had no PAB positive .The speci‐ficity and sensitivity of ASCA (+ )in the CD group were 96 .53% and 18 .75% respectively ;which in the UC group were 96 .37%and 53 .33% respectively .Conclusion The serum antimicrobial antibody detection can provide a basis for clinical diagnosis of in‐flammatory bowel disease .