首页 | 本学科首页   官方微博 | 高级检索  
检索        

JNK信号通路活化与BCMA表达对MM细胞的作用研究
引用本文:徐光,班永宏,鞠少卿,朱宝立.JNK信号通路活化与BCMA表达对MM细胞的作用研究[J].国际检验医学杂志,2016(22).
作者姓名:徐光  班永宏  鞠少卿  朱宝立
作者单位:1. 江苏省疾病预防控制中心,南京,210028;2. 江苏省南通大学附属医院医学检验中心 226001
摘    要:目的研究多发性骨髓瘤(MM)细胞中B细胞活化因子(BAFF)及B细胞成熟抗原(BCMA)的表达及作用,JNK信号通路的表达及活化情况,探讨JNK信号通路活化与BCMA表达间的相互作用。方法采用实时荧光定量(QRT)-聚合酶链式反应(PCR)检测MM患者及健康对照者单核细胞(PBMCs)中BAFF及其受体BCMA、TACI、BAFF-R的表达。采用Western Blot方法检测MM细胞中BCMA蛋白的表达及信号通路蛋白ERK、p-ERK、JNK、p-JNK、p38及p-p38的表达及活化情况;采用WST-1法检测MM细胞的增殖与存活。结果 MM患者PBMCs中BAFF及BCMA的表达显著高于健康对照者。人BAFF重组体(rhBAFF)促进MM细胞的增殖,Si-BCMA可抑制rhBAFF对MM细胞的增殖作用。除了ERK、JNK及p38的表达外,MM细胞中还有活化蛋白p-JNK的表达。Si-BCMA可下调p-JNK的表达,JNK信号通路抑制剂可下调p-JNK及BCMA的表达。结论 MM细胞中,BAFF通过BCMA促进MM细胞增殖。JNK信号通路活化与BCMA表达相互作用,共同促进MM细胞的增殖与存活。

关 键 词:多发性骨髓瘤  B细胞活化因子  B细胞成熟抗原  JNK信号通路

Interaction between JNK signal pathway activation and BCMA expression collectively promotes the survival of multiple myeloma cells
Abstract:Objective To investigate the expression and role of B‐cell activating factor (BAFF) and B‐cell maturation antigen (BCMA) in multiple myeloma(MM) cells ,expression and activation of JNK signal pathway and to explore the interaction between JNK signal pathway activation and BCMA expression .Methods The expression of BAFF and its receptor BCMA ,TACI and BAFF‐R in peripheral blood mononuclear cells(PBMCs) of MM patients and healthy controls were detected by the quantitative real‐time fluorescence PCR(QRT‐PCR) .Western blot was adopted to detect the expression of BCMA protein and the expression and ac‐tivation of signal pathway protein ERK ,p‐ERK ,JNK ,p‐JNK ,p38 and p‐p38 .MM cell proliferation and survival were measured by WST‐1 assay .Results The expression of BAFF and BCMA in PBMCs of MM patients was significantly higher than that of healthy controls .Recombinant human BAFF(rhBAFF) promoted the proliferation of MM cells ,while Si‐BCMA could restrain the prolifera‐tive effect of rhBAFF on MM cells .In addition to the expression of ERK ,JNK ,p38 ,p‐JNK was also expressed in MM cell lines .Si‐BCMA could down‐regulate the expression of p‐JNK ,while the JNK pathway inhibitor could down‐regulate the expression of p‐JNK and BCMA .Conclusion BAFF promotes the proliferation of MM cells via BCMA .The interaction between JNK signal path‐way activation and BCMA expression commonly promotes the proliferation and survival of MM cells .
Keywords:multiple myeloma  B cell-activating factor  B-cell maturation antigen  JNK signal pathway
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号