成纤维样滑膜细胞原代培养方法的优化

目的 对优化佐剂型关节炎(AA)大鼠成纤维样滑膜细胞(FLSs)的原代培养方法并进行鉴定.方法 制备AA大鼠模型,采用酶消化法分离得到滑膜细胞,再经多次消化后重贴壁得到FLSs,并通过免疫荧光技术鉴定.结果 多次实验均成功培养出FLSs,且经鉴定的所有细胞几乎全为vimentin阳性.结论 本实验方法成功培养出高纯度的FLSs,为以滑膜为靶点的类风湿性关节炎(RA)的研究奠定了基础.

The optimization of fibroblast-like synoviocytes' primary culture

Objective The present study aims to improve the primary culture of fibroblast-like synoviocytes (FLSs) of AA rats and identify them.Methods The adjuvant-induced arthritis model of rats was prepared.Synovial cells were isolated by enzyme digestion method.After multiple processes of digestion,readhering FLSs were obtained.FLSs that specifically express vimentin were identified by immunofluorescence.Results FLSs were successfully cultured by this method for multiple times,and almost all cells were identified as FLSs.Conclusion This method could be used for high-purity primary culture of FLSs and it can lay foundations for further studies targeting synovial membrane in rheumatoid arthritis (RA).