酪氨酸激酶受体B和脑源性神经营养因子在宫颈癌及癌旁组织中的表达差异

目的：研究酪氨酸激酶受体B（TrkB）和脑源性神经营养因子（BDNF）在宫颈癌组织、癌旁组织和正常宫颈组织中的表达情况，分析其与宫颈癌临床病理特征的关系，探讨癌周组织细胞凋亡信号通路的激活与肿瘤恶性程度的关系。方法选择宫颈浸润癌患者72例，以同期因良性病变行子宫切除的正常宫颈组织72例为对照。构建包括宫颈癌组织、癌旁组织、正常宫颈组织在内的组织芯片，采用免疫组化法检测TrkB、BDNF表达情况，比较上述指标在不同组织中的表达差异。结果共构建组织芯片6张。 TrkB和BDNF在宫颈癌原发灶中的表达率分别为70.80%和91.70%，明显高于癌旁组织的25.00%和48.60%，正常宫颈组织几乎不表达（阳性率分别为4.20%和1.40%）。 TrkB在宫颈癌组织中的阳性率与有无脉管侵犯及淋巴结转移有关（χ2=12.50和12.36，P<0.01），与临床分期、病理分级、肿瘤直径、浸润深度的关系不明显（P均>0.05），而BDNF总体阳性率高，其表达与上述所有因素的相关性均不明显（P均>0.05）。癌旁组织中TrkB、BDNF表达与肿瘤直径、是否存在脉管浸润及淋巴转移有关（P<0.01或P<0.05）。结论癌旁组织中存在一定量的TrkB、BDNF表达，其表达强度与肿瘤直径、有无脉管浸润及淋巴转移显著相关，且比肿瘤组织更能反映其恶性程度。癌周组织TrkB、BDNF表达也许可作为新的评价宫颈癌患者预后的生物学指标。

Different expressions of tyrosine kinase B and brain-derived neurotrophic factor in cervical cancer and ;paracancerous tissue

Objective To study tyrosine kinase B (TrkB) and brain-derived neurotrophic factor (BDNF) expression in cervical carcinoma tissue, paracancerous tissues and normal cervical tissue,analyze their relationship with clinical pathological characteristics of cervical cancer, and to investigate the relation between apoptosis signal pathway activation around cancer cells and tumor malignancy. Methods A total of 72 cases with cervical invasive carcinoma were selected, while 72 cases of normal cervical tissues were selected as control group. Tissue microarrays of cervical cancer tissues, adjacent tissues and normal cervical tissues were made and the expressions of TrkB and BDNF were detected and compared by immunohistochemistry method. Results A total of 6 tissue chips were constructed. Expression rates of TrkB and BDNF in cervical cancer were 70.80% and 91.70%, respectively, and were significantly higher than those in paracancerous tissue (25.00% and 48.60%). There was almost no expression in normal cervical tissue, of which the positive rates were 4.20% and 1.40%. The positive rate of TrkB in cervical cancer was related to vascular invasion and lymph node metastasis (χ2=12.50 and 12.36, P<0.01), and had no correlation with clinical stage, pathological grade, tumor diameter and infiltration depth (P all>0.05). Meanwhile, the total positive rate of BDNF was high, and had no correlation with all factors above (P all>0.05). The expressions of TrkB and BDNF in cancer adjacent tissue were related to the tumor diameter, vascular invasion and lymph node metastasis (P<0.01 or P<0.05). Conclusions There are some TrkB and BDNF expressions in the cervical cancer adjacent tissues. The expression levels in paracancerous tissues are significantly correlated with tumor diameter, vascular invasion and lymph node metastasis, and show better reflection of the malignant degree than those in tumor tissues. The expressions of TrkB and BDNF in paracancerous tissues may be used as new biological markers for evaluating the prognosis of patients with cervical cancer.