| 间充质干细胞对睾丸辐射损伤的影响 |
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| 引用本文： | 郭洪波,蒋玲,孟伟,郭坤元. 间充质干细胞对睾丸辐射损伤的影响[J]. 实用医学杂志, 2011, 27(15). DOI: 10.3969/j.issn.1006-5725.2011.15.009 |
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| 作者姓名： | 郭洪波 蒋玲 孟伟 郭坤元 |
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| 作者单位： | 1. 南方医科大学珠江医院,广州市,510282
2. 南方医科大学基因工程研究所,广州市,510515 |
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| 摘 要： | 目的:研究间充质干细胞(MSCs)对睾丸辐射损伤的影响,为临床上治疗放射性睾丸损伤奠定理论基础.方法:选取15只雄性SD大鼠随机分为对照组、模型组和治疗组.模型组和治疗组大鼠均接受直线加速器产生的高能电子线行一次性全身照射;对照组不接受照射.治疗组大鼠接受辐射24 h后尾静脉输注MSCs 3 × 106 /只;模型组和对照组大鼠尾静脉输注生理盐水1 mL;共3周.当模型组处于濒死状态时处死所有大鼠,检测各组大鼠血清中睾酮水平、血清和睾丸组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性;采用苏木精-伊红染色法 (HE染色法)制作大鼠的睾丸病理切片,观察辐射对睾丸形态学的影响.结果:模型组大鼠精神萎靡,反应迟钝,活动少,嗜睡,毛发变黄;治疗组大鼠较模型组反应灵敏.治疗组大鼠血清SOD明显高于模型组,而MDA水平低于模型组;治疗组大鼠血清睾酮含量明显高于模型组,但较正常组低.病理结果显示对照组生精小管横切面见精原细胞及不同发育阶段的精母细胞、精子细胞、精子,组成多种形态的完整生精上皮图像;模型组生精上皮变薄,生精层数减少,各级生精细胞明显减少,部分曲细精管内仅剩支持细胞和少量精原细胞,精子细胞及精子消失,显示辐射对大鼠睾丸组织损伤明显;治疗组外形较规则,各级生精细胞有序排列,初级精母细胞、精子细胞数量增多,精子发生良好,形态结构接近于对照组.结论:MSCs对睾丸的辐射损伤具有明显的修复作用,能够改善睾丸的生殖功能.
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| 关 键 词： | 间充质干细胞 辐射损伤 睾丸 |
Mesenchymal stem cells on radiation-induced testicular injury |
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| GUO Hong-bo,JIANG Ling,MENG Wei,GUO Kun-yuan. Mesenchymal stem cells on radiation-induced testicular injury[J]. The Journal of Practical Medicine, 2011, 27(15). DOI: 10.3969/j.issn.1006-5725.2011.15.009 |
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| Authors: | GUO Hong-bo JIANG Ling MENG Wei GUO Kun-yuan |
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| Affiliation: | GUO Hong-bo*,JIANG Ling,MENG Wei,GUO Kun-yuan. *Zhujiang Hospital of Southern Medical University,Guangzhou 510282,China. |
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| Abstract: | Objective To investigate the effect of mesenchymal stem cells (MSCs) on testis of rats with irradiation injury, and to provide the theoretical foundation of clinical treatment for radiation-induced testicular injury. Methods Fifteen male SD rats were randomly divided into 3 groups , namely control group, model group, and treatment group. Rats in the model group and treatment group received one-time total body irradiation with electron beam generated by high-energy linear accelerator line , 6 Gy per week, a ... |
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| Keywords: | Mesenchymal stem cell Radiation damage Testicle |
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