| Blockage of PPARδ increases the expression of inflammatory factors in 3T3-L1 cells stimulated with TNFα |
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| 引用本文： | 张莉莉,祝之明,曹廷兵,王利娟.Blockage of PPARδ increases the expression of inflammatory factors in 3T3-L1 cells stimulated with TNFα[J].中国人民解放军军医大学学报,2006(2). |
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| 作者姓名： | 张莉莉 祝之明 曹廷兵 王利娟 |
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| 作者单位： | Department of Endocrinology Institute of Surgery Research，Daping Hospital，Third Military Medical University，Chongqing 400042，China，Department of Endocrinology，Institute of Surgery Research，Daping Hospital，Third Military Medical University，Chongqing 400042，China，Department of Endocrinology，Institute of Surgery Research，Daping Hospital，Third Military Medical University，Chongqing 400042，China，Department of Endocrinology，Institute of Surgery Research，Daping Hospital，Third Military Medical University，Chongqing 400042，China |
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| 摘 要： | Objective: To investigate the role of peroxisome proliferator-activated receptors δ (PPARδ) in inflammatory reaction and its possible mechanism in adipocyte. Methods :Lentivirus-mediated RNA interference (RNAi) was used to block the expression of PPARS in 3T3-L1 cells. In order to induce inflammation in 3T3-L1, cells were stimulated with tumor necrosis factor-α(TNFα, 20 ng/ml) for 4 h. The expression of PPAR8, nuclear factor κB (NFκB) and C reactive protein (CRP) were determined by Western blot analysis. Results:The expression of PPARδ was reduced by 80% after RNAi. Blockage of PPARδ promoted the expression of CRP and NFκB in cells stimulated with TNFα, but had no effect on normal cells. Conclusion: PPARδ is involved in inflammatory reaction in adipocyte. Blockage of PPARδ can promote the inflammation mediated by inflammatory factors and increase the expression of NFκB and CRP in 3T3-L1 cells stimulated with TNFα.
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| 关 键 词： | RNA interference 3T3-L1 cells peroxisome proliferator-activated receptors δ nuclear factor κB C reactive protein |
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