晚期糖基化终产物对人脂肪间充质干细胞功能影响的体外研究*

 目的：探讨晚期糖基化终产物（AGEs）对人脂肪来源的间充质干细胞（hADSCs）促进创伤修复功能的影响。方法：体外培养hADSCs，实验分为牛血清白蛋白（BSA）对照组、低浓度糖基化修饰的牛血清白蛋白（AGE-BSA）效应组和高浓度AGE-BSA效应组。采用WST法和Transwell迁移实验检测各组细胞增殖和迁移情况。应用实时定量PCR和ELISA检测各组细胞分泌血管内皮生长因子（VEGF）、肝细胞生长因子（HGF）和胰岛素样生长因子1（IGF-1）的表达。结果：与对照组相比， AGE-BSA组增殖能力和迁移能力明显下降（P<0.05），VEGF、HGF和IGF-1 mRNA及蛋白表达水平显著降低（P<0.05）。结论：AGEs能损伤hADSCs的促进创伤修复功能，因而能够影响hADSCs治疗糖尿病皮肤溃疡病的治疗效果。

Effect of advanced glycosylation end products on function of human adipose tissue-derived stem cells in vitro

AIM：To explore the effect of advanced glycosylation end products (AGEs) on the function of human adipose-derived stem cells (hADSCs) in promoting wound healing. METHODS：hADSCs were isolated by conventional method in vitro and divided into control bovine serum albumin (BSA) group, low-dose AGE-BSA group and high-dose AGE-BSA group. The proliferation and migration of hADSCs with different treatments were determined by WST-8 assay and Transwell assay, respectively. In addition, the expression of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and insulin-like growth factor-1(IGF-1) at mRNA and protein levels was determined by real-time PCR and ELISA analysis. RESULTS：Compared with control group, the proliferation and migration abilities were significantly inhibited in the hADSCs of AGE-BSA group. The mRNA expression of VEGF, HGF and IGF-1 in AGE-BSA group was obviously lower than that in control group. The contents of VEGF, HGF and IGF-1 in hADSCs-conditioned me-dium in AGE-BSA group were also obviously lower than those in control group. CONCLUSION：AGEs alter the intrinsic properties of hADSCs and impair their functions in promoting wound healing, thus affecting the therapeutic potential of hADSCs in the treatment of diabetic ulcers.