吉西他滨联合卡铂诱导NK/T细胞淋巴瘤细胞株凋亡的研究

目的:研究吉西他滨联合卡铂对NK/T细胞淋巴瘤细胞株(SNK 6)细胞增殖及凋亡的影响。方法:采用细胞增殖-毒性检测法(CCK-8)检测吉西他滨、卡铂、顺铂对SNK 6细胞增殖的影响,算出各自半数抑制浓度(IC 50)值;再用吉西他滨与卡铂或顺铂做联合实验,用金氏公式计算Q值,评价联合用药效应。应用流式细胞仪检测不同药物组对细胞凋亡的影响。蛋白免疫印迹法(Western Blotting)检测各药物组Cleaved-caspase-3、B淋巴细胞瘤-2蛋白(Bcl-2)、Bcl-2相关X蛋白(Bax)等凋亡蛋白的表达水平。结果:吉西他滨、卡铂、顺铂对SNK 6细胞均有较好的抑制作用;联合用药中,吉西他滨+卡铂组与吉西他滨+顺铂组对SNK 6细胞抑制作用相当,两药联合为相加作用。吉西他滨+卡铂组凋亡率稍低于吉西他滨+顺铂组,但差异无统计学意义(P>0.05)。吉西他滨可上调Cleaved-caspase-3蛋白、Bax蛋白的表达水平,下调Bcl-2蛋白的表达水平(P<0.05),吉西他滨与卡铂或顺铂联合时效果更加显著。结论:在体外环境下,吉西他滨联合卡铂可抑制SNK 6细胞增殖并诱导其凋亡,且一定浓度的吉西他滨联合卡铂对SNK 6细胞株的凋亡率与吉西他滨联合顺铂相似。

Study on the apoptosis of NK/T cell lymphoma cell line induced by gemcitabine combined with carboplatin

Objective:In order to investigate the proliferation and apoptosis effects of gemcitabine combined with carboplatin on NK/T cell lymphoma SNK 6 cells.Methods:The CCK-8 method was used to detect the proliferation of SNK6 cells treated with gemcitabine,carboplatin and cisplatin,calculate the half-inhibitory concentration(IC 50)of the three drugs.Then,gemcitabine was combined with carboplatin or cisplatin to test the proliferation ability of SNK 6 cells.The Q value was calculated by the Gold's formula and was used to evaluate the combined drug effect.The effects of each drug group on apoptosis were detected by flow cytometry.The expression of proteins Bcl-2,Bax and Cleaved-caspase-3 in each drug group was detected by western blotting.Results:Gemcitabine,carboplatin and cisplatin have significant inhibitory effects on SNK 6 cells.In the combination,the gemcitabine+carboplatin group and the gemcitabine+cisplatin group had similar inhibitory effects on SNK 6 cells.The combination of the two drugs are additive.The apoptosis rate of gemcitabine+carboplatin group was slightly lower than that of gemcitabine+cisplatin group,the difference was not statistically significant(P>0.05).The effect of gemcitabine could down-regulate the expression of Bcl-2 protein and up-regulate the expression of Cleaved-caspase-3 protein and Bax protein(P<0.05).The effect of gemcitabine combined with cisplatin and carboplatin was more significant.Conclusion:In vitro,carboplatin combined with gemcitabine can inhibit the proliferation and induce apoptosis of SNK 6 cells,and carboplatin combined with gemcitabine can achieve similar apoptotic rate of cisplatin combined with gemcitabine in SNK 6 cell line.