Nanotechnology in ligature-induced periodontitis: protective effect of a
doxycycline gel with nanoparticules |
| |
Authors: | Marco Antonio BOTELHO Jose Galberto MARTINS Ronaldo Sousa RUELA Dinalva Brito QUEIROZ Wagner Sousa RUELA |
| |
Institution: | 1. Post Doctor in Orthodontics, Federal University of Ceará, Fortaleza, CE, Brazil.;2.Doctor in Chemistry, Regional University of Cariri, Fortaleza, CE, Brazil.;3.Doctor in Dentistry, Scholar of National Research and Development Council CNPq, FUNORTE School of Dentistry, Montes Claros, MG, Brazil.;4.Scholar of National Research and Development Council CNPq, Fortaleza, CE, Brazil. |
| |
Abstract: | ObjectivesThe aim of this study was to test the efficacy of a locally applied 8.5%
nanostructured doxycycline (DOX) gel in preventing alveolar bone loss in
experimental periodontal disease (ePD) in rats by using the tapping mode atomic
force microscopy (AFM).Material and MethodsePD was induced in 24 Wistar rats. Animals were treated with the doxycycline gel
topically, immediately after ePD induction, and 3 times a day during 11 days. Four
groups (n=6) were formed as follows: Naïve group (animals not subjected to
ePD nor treated); non-treated (NT) group (animals subjected to ePD, but not
treated); vehicle gel (VG) group (animals subjected to ePD and treated with
topical gel vehicle); and DOX group (test group): animals subjected to ePD and
treated with the 8.5% DOX gel. In order to investigate topographical changes in
histological sections, a novel simple method was used for sample preparation, by
etching sections from paraffin-embedded specimens with xylol.ResultsComparing the AFM images, several grooves were observed on the surface of the
alveolar bone and other periodontal structures in the NT and VG groups, with
significantly greater depths when compared to the DOX group (p<0.05).ConclusionsPeriodontal structures were brought into high relief confirming to be a simple and
costeffective method for AFM imaging with ultrastructural resolution. The
doxycycline gel was able to afford periodontal surface preservation, with flatter
grooves. |
| |
Keywords: | Nanotechnology Doxycycline Periodontitis Atomic force microscopy Gel |
|
|