腺样囊性癌高、低转移细胞株基因表达谱差异性及转移相关基因研究

目的　试图找出与涎腺腺样囊性癌转移相关的基因。方法　应用抑制性消减杂交方法研究这两个细胞株基因表达上的差异。结果　相对于驱动子高转移细胞株ACC M ,在测试子低转移细胞株ACC 2中有 12个基因呈高表达。获得的基因序列中包括 10个已知序列及 2个新序列。新发现的 2个基因序列分别是血管生成抑制因子同源的囊性癌转移相关基因 (ACCmetastasis associatedRNH)和囊性癌转移相关蛋白 (ACCmetastasis associatedsuspectedprotein) ,已被GenBank登录 (登录号分别为AF5 2 2 0 2 4和AF5 2 2 0 2 5 )。结论　ACC M中部分基因的低表达或突变与肿瘤高转移特性的获得有关 ,为进一步探索该肿瘤转移控制和基因治疗提供了重要的分子生物学资料

Gene expression differences between high and low metastatic cells of Adenoid cystic carcinoma

Objective To investigate the complex differences between high metastatic and low metastatic cells of the Adenoid cystic Carcinoma. Methods Gene expression patterns were examined in high metastatic cell ACC M strain and low metastatic ACC 2 strain with the method SSH (suppression subtractive hybridization).Results although extensive similarity was noted between the expression profiles,twelve genes were highly expressed of,in low metastatic cell ACC 2 tester,compared with driver,high metastatic cell ACC M.These genes were cysteine rich angiogenic inducer protein (cyr61),chromosome7 clone RP11 52501,G protein,was family member Iferritin heavy polypeptide I,jumping translocation breakpoint,eukaryotic translation elongation,folate receptor,ribosomal proteins L7a, S21,P0 and other two novel genes ACC metastasis associated RNH and ACC metastasis associated suspected protein .GenBank accession number were AF522024 and AF522025 respectively .Conclusions the result suggests that the obtainment of the ability of metastasis is related to the low expression or mutation of these genes .These data provide insight into the extent of expression differences underlying metastasis related genes that may prove useful as diagnostic or prognostic markers.