镁对缺氧大鼠皮质神经元保护作用的研究

目的研究镁对离体培养的缺氧大鼠脑皮质神经元的影响。方法将大鼠皮质神经元分别给予MgSO4和Mg^2＋-ATP处理后连续给予97．5％N2和2．5％CO2混合气体，制成大鼠皮质神经元体外缺氧试验模型，分别观察缺氧后1、2、4h不同时间神经元死亡率、神经细胞内Ca^2＋浓度（Fluo-3标记，激光共聚焦显微镜检测）的动态变化和培养液中LDH、K^＋、NSE浓度的变化。结果MgSO4和Mg^2＋-ATP能减少缺氧状态下的脑皮质神经元钙内流（P〈0．05），降低培养液中LDH、K^＋、NSE浓度（P〈0．05），明显降低神经元死亡率（P〈0．05）。结论镁对离体培养的皮质神经元缺氧损伤有肯定的保护作用。

A Study on the Protective Efficacy of Magnesium Against Anoxic Damage to Cultured Neurons of Rats

Objective This is an in vitro study aimed to assess the influence of magnesium on cultured anoxic cortical neurons of rats. Methods After being treated with MgSO_4 and Mg~ 2+ -ATP respectively, the cortical neurons of rats were given mixture of 97.5% N_2 and 2.5% CO_2 continuously to set up the model of anoxic cortical neuron of rats at different time points. The mortality of neurons, the levels of Ca~ 2+ within nerve cells (marked by Fluo-3, detected by laser confocal microscopy), and the levels of lactate dehydrogenase (LDH), K~+ and neuron specific enolase(NSE) in the culture fluid were investigated at 1,2,4 hours after the inception of anoxia. Results MgSO_4 and Mg~ 2+ -ATP reduced the calcium influx of anoxic cortical neuron ( P<0.05), lowered the level of LDH, K~+, NSE in the culture fluid (P<0.05) and significantly decreased the mortality of neurons( P<0.05). Conclusion The protective efficacy of magnesium against the anoxic damage to cultured cortical neurons of rats is corroborated.