人晶状体上皮细胞凋亡时细胞外信号调节激酶表达的变化

目的研究紫外线B引起培养的人晶状体上皮细胞凋亡时细胞外信号调节激酶(ERK)蛋白表达水平的变化。方法以人晶状体上皮细胞系为研究模型,其中实验组用紫外线B照射后与对照组继续培养12h,然后通过蛋白印迹(Westernblot)法检测两组ERK酶表达的量。结果试验组上样品量明显少于对照组,而两组ERK1/2表达的量相等,故说明实验组实际ERK1/2表达量强于对照组。结论人晶状体上皮细胞在紫外线照射下发生凋亡,此时ERK1/2表达量随之增强。该研究为进一步深入研究ERK1/2在白内障和后发障形成过程中的作用打下良好基础。

Changes in extracellular-signal regulated protein kinase expression between normal and apoptotic lens epithelial cells cultured

[Objective] To use UVB-irridatiaon to cause apoptosis of cultured human lens epithelial cells(LECs),and to observe the changes in extracellular-signal regulated protein kinase(ERK) expression between normal and apoptotic LECs.[Methods] Cultured LECs were involved in this study.The test groups were irridated with UVB.And then the test and control groups were cultured for 12 hours.The expression of ERK of all the groups was detected using Western Blot method.[Results] The cells of the test group was half of that of the control group,but the expressions of ERK in the two groups were the same.Thus the expressions of ERK in the test groups were more than that in the control groups.[Conclusion] The UVB-irridatiaon can cause apoptosis of cultured LECs,when the expressions of ERK of the LECs increase.This study is useful to further research of ERK in formation of cataract and posterior capsular opacification.