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HPLC法测定银花抗感片中绿原酸的含量
引用本文:郭霞,吴庆娜,宫钦红. HPLC法测定银花抗感片中绿原酸的含量[J]. 齐鲁药事, 2005, 24(5): 283-284
作者姓名:郭霞  吴庆娜  宫钦红
作者单位:烟台市毓璜顶医院,烟台,264000
摘    要:
目的采用高效液相色谱法测定银花抗感片中绿原酸的含量。方法色谱柱C18,以乙腈-0.4%磷酸溶液(10∶90)为流动相;检测波长为327nm。结果绿原酸的进样量在0.04~0.8μg范围内与峰面积呈良好的线性关系(r=0.9996),平均加样回收率为98.1%,RSD为1.15%。结论该方法简便易行,结果可靠,可有效地控制银花抗感片的质量。

关 键 词:银花抗感片  金银花  绿原酸
文章编号:1672-7738(2005)05-0283-02

Determination of Chlorogenic acid in Yinhuakanggan Tablets by HPLC
Guo Xia,Wu Qing-na,Gong Qin-hong. Determination of Chlorogenic acid in Yinhuakanggan Tablets by HPLC[J]. qilu pharmaceutical affairs, 2005, 24(5): 283-284
Authors:Guo Xia  Wu Qing-na  Gong Qin-hong
Abstract:
Objective To determine the Chlorogenic acid in Yinhuakanggan Tablets by HPLC.Methods C_18 column was used.Acetonitrile-0.4% phosphoric acid (10∶90)was as mobile phase.Detective wavelength was at 327nm.Results Chlorogenic acid which was at 0.04~0.8μg has good linear relation with peak area(r=0.9996),average recovery was 98.1% and RSD was 1.15%.Conclusion The method was convenient and the result was reliable.It can control the quality of Yinhuakanggan Tablets efficiently.
Keywords:Yinhuakanggan Tablets  Honeysuckle flower  Chlorogenic acid
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