霍乱弧菌不同群体菌株的群体遗传特征比较

应用ＭＥＥ法对５９２株霍乱弧菌的１５个酶基因位点进行检测，分析和比较霍乱弧菌不同群体的群体遗传特征。结果：古典型菌株（ＣＶＣ）、埃尔托流行株（ＥＳＥＶＣ）和Ｏ１３９菌株（Ｏ１３９）的群体平均遗传多态值和平均杂合度都很低，ＨＣＶＣ＝０．１０６、ｈＣＶＣ＝１．５，ＨＥＳＥＶＣ＝０．０５６、ｈＥＳＥＶＣ＝２．３（Ｏ１３９包含在ＥＳＥＶＣ之中），被检测的基因位点多为非多态位点；而同为Ｏ１群菌的埃尔托非流行株（ＮＳＥＶＣ）的平均遗传多态值和平均杂合度要高得多，接近非Ｏ１群菌株（ＮＯ１）的群体遗传学指标，分别为ＨＮＳＥＶＣ＝０．４２５、ｈＮＳＥＶＣ＝５，ＨＮＯ１＝０．６２７、ｈＮＯ１＝６．５，被检测的基因位点几乎全是遗传多态位点。结果表明：ＥＳＥＶＣ和ＮＳＥＶＣ虽然都是Ｏ１群菌，但在群体遗传学特征上存在巨大差异；ＣＶＣ、ＥＳＥＶＣ和Ｏ１３９为遗传学性状稳定、群体遗传结构紧密的群体，而ＮＳＥＶＣ和ＮｏｎＯ１的群体遗传关系离散

Comparison on the population genetic characteristics of different groups of Vibrio cholerae

In order to study the epidemiological significance and population genetics of Vibrio cholerae (VC), here we reported the results of 592 isolates of different groups of VC depending on 15 gene loci with multilocus enzyme electrophoresis. The results showed that the average genetic diversities and heterozygosities in CVC (Classical strains), ESEVC(epidemigenic strains of El Tor) and O139 were very low: H CVC = 0.106 , h CVC =1.5; H ESEVC =0.056, h ESEVC =2.3(including O139); most of the gene loci detected were not diversified. Meanwhile the average genetic diversity and heterozygosity of NSEVC (non epidemigenic strains of El Tor), were near to those of VC non O1: H NSEVC =0.425, h NSEVC =5; H Non O1 = 0.627 , h Non O1 =6.5;The gene loci detected in these strains were almost diversified. These results indicated that the pathogens of cholera (including CVC, ESEVC and O139) were stable in genetic characteristics and the relationships among these strains were close. But the relationships among NSEVC, even belonging to VC O1, and non O1 strains were disperse.