| 卡氏肺孢子虫肺炎动物模型建立及其检测方法 |
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| 引用本文： | 瞿介明,李锡莹,何礼贤,胡必杰,潘文生,周燕南,周春妹. 卡氏肺孢子虫肺炎动物模型建立及其检测方法[J]. 复旦学报(医学版), 1992, 0(3) |
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| 作者姓名： | 瞿介明 李锡莹 何礼贤 胡必杰 潘文生 周燕南 周春妹 |
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| 作者单位： | 上海医科大学中山医院肺病学教研室(瞿介明，李锡莹，何礼贤，胡必杰)，上海医科大学中山医院外科病理室(潘文生，周燕南)，上海医科大学中山医院肺病学教研室(周春妹) |
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| 摘 要： | 用皮下注射醋酸可的松法诱导大鼠卡氏肺孢子虫肺炎(PCP)模型,成功率20％。对PCP模型大鼠(n=6)进行诊断采样,结果:肺病理学检查卡氏肺孢子虫(PC)检出率为100％(6/6)。用经支气管肺活检(TBLB)法临床诊断1例早期PCP。支气管肺泡灌洗液(BALF)涂片和肺组织印片的姬姆萨染色,可见PC囊内小体及滋养体。改进的哥氏银染适用于肺组织切片,包囊壁浓染呈黑色,易于识别。对于免疫功能低下怀疑为PCP患者,应尽早作TBLB和(或)BAL检查,可以达到早期诊断。
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| 关 键 词： | 卡氏肺孢子虫肺炎 动物模型 检测方法 |
THE PRODUCTION OF AN ANIMAL MODEL OF PNEUMOCYSTIS CARINII PNEUMONIA AND IMPROVEMENT OF METHOD OF IDENTIFICATION |
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| Qu Jiemiag,Li Xiying,He Lixian,Hu Bijie,Pan Wenshen,Zhou Yannan,Zhou Ohenmei. THE PRODUCTION OF AN ANIMAL MODEL OF PNEUMOCYSTIS CARINII PNEUMONIA AND IMPROVEMENT OF METHOD OF IDENTIFICATION[J]. Fudan University Journal of Medical Sciences, 1992, 0(3) |
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| Authors: | Qu Jiemiag Li Xiying He Lixian Hu Bijie Pan Wenshen Zhou Yannan Zhou Ohenmei |
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| Abstract: | A rat model of Pneumocystis oarinii pneumonia (PCP) was established by fluboutaneous injections of cortisone acetate. The total positive provocation rate was 20%, resulting in 6 wistar with pop. Various lung histopathological specimens were studied, all were positive (6/6). One clinical case of early POP was diag- nosed successfully by transbronchial lung biopsy (TBLB). Giemsa stain was used on smears obtained from bronchial alveolar lavage fluids (BALF) and on lung imprints, intraoystio bodies and trophozoites of Pneumooystis carinii were revealed apparently. Modified Gomori-Grocott's methenamine silver nitrate stain is suitable for pulmonary histopathological slides, as oyst walls of Pneumooystis carinii stained black and could be easily recognized. TBLB and/or BAL should be performed as early as possible in immunocompromised patients with suspected PCP, for an early diagnosis of PCP. |
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| Keywords: | pneumoeystis oarinii pneumonia animal model identification method |
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