针刺对多囊卵巢综合征大鼠不孕的影响

目的 观察针刺对多囊卵巢综合征（PCOS）大鼠不孕的影响, 并探讨其作用机制。

Influences of Acupuncture on Infertility of Rats with Polycystic Ovarian Sydrome

Objective To observe the effect and mechanism of acupuncture on infertility of rats with polycystic ovarian syndrome （ PCOS）. Methods PCOS rat model was induced by subcutaneous injection of oil soluion of dehydroepiandrosterone （DHEA） in immature （24-day-old） female rats for continuous 20 days. Rats for control were given with same dose of oil for instead. PCOS rats were randomly divided into the model group un- treated and the acupuncture group treated by needling acupoints of Guanyuan （CV4）, Zhongji （CV3）, Sanyinjiao （SP6） and Zigong （CX-CA1）, 15 min once a day for 5 continuous days, starting from the 80th day after birth. All rats were sacrificed at terminal of the treatment, their uterus and bilateral ovaries were dissected for ob- servation and blood levels of sex hormones were measured. Results Compared with the model group, the num- ber of implanted blastocyte and blastocyte implantation rate were higher and the blood levels of testosterone （T） and estradiol （ E2 ） were lower in the acupuncture group （ P 〈0.05） ; but the difference between groups in serum levels of follicular stimulating hormone, luteinzing hormone and progesterone were of statistical insignificance （P 〉0.05）. Moreover, the wet weight of ovary was lower and the equipotent diameter and area of glandular organ and cavity, area ratio of gland and the stroma, and mean thickness of endometria were higher in the acu- puncture group than those in the control group （ P 〈0.05）. Conclusion Acupuncture can conspicuously downregulate the expressions of serum levels of T and E2 , improve the development of ovaries and uterus, promote ovulation, enhance endometrial receptivity, and advance blastocyte implantation.