Differential localisation of the metabotropic glutamate receptor mGluR1a and the ionotropic glutamate receptor GluR2/3 in neurons of the human cerebral cortex |
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| Authors: | W Y Ong Y He K K Tan L J Garey |
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| Institution: | (1) Department of Anatomy, National University of Singapore, Singapore 119260 e-mail: antongwy@us.edu.sg, Fax: +65-778-7643, SG;(2) Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York NY 10029, USA, US;(3) Department of Neurosurgery, Tan Tock Seng Hospital, Singapore 308433, SG;(4) Division of Neuroscience, Imperial College School of Medicine, Charing Cross Hospital, London W6 8RF, UK, GB |
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| Abstract: | Specimens of human cerebral cortex were obtained during neurosurgical operations and studied by immunocytochemistry and electron
microscopy, using antibodies to the metabotropic glutamate receptor subunit mGluR1a and the ionotropic glutamate receptor
GluR2/3. A small number of non-pyramidal neuronal cell bodies were labelled for mGluR1a. Double immunolabelling with mGluR1a
and GluR2/3 showed that most pyramidal cell bodies were labelled for GluR2/3 but not for mGluR1a. Despite the non-colocalisation
of these two receptor subtypes in cell bodies, however, many dendrites and dendritic spines were double-labelled for mGluR1a
and GluR2/3 at electron microscopy. As there is evidence that most neurons positive for GluR2/3 are pyramidal cells, this
suggests that mGluR1a is present in dendrites of pyramidal neurons, despite absent or low levels of immunoreactivity in their
cell bodies.
Received: 5 May 1997 / Accepted: 24 July 1997 |
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| Keywords: | Glutamate receptors Immunocytochemistry Electron microscopy Human cortex |
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