缺血性急性肾功能衰竭与细胞凋亡

目的：探讨缺血性急性肾功能衰竭（ＩＡＲＦ）与细胞凋亡的关系以及褪黑素（ＭＥＬ）对ＩＡＲＦ的保护作用。方法：用无损伤动脉夹钳夹大鼠双侧肾蒂４５ｍｉｎ再灌注２４ｈ的手术方法制成ＩＡＲＦ动物模型，观察不同剂量ＭＥＬ预防用药对ＩＡＲＦ大鼠血尿素氮（ＢＵＮ）、肌酐（Ｃｒ）、超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨ Ｐｘ）、丙二醛（ＭＤＡ）的影响以及对肾小管细胞凋亡的影响。结果：ＩＡＲＦ大鼠ＢＵＮ，Ｃｒ升高，ＳＯＤ，ＧＳＨ Ｐｘ下降，ＭＤＡ上升。形态学显示肾小管细胞出现细胞凋亡。ＭＥＬ能不同程度地逆转上述改变，防止或减少凋亡发生，效应呈剂量依赖性。结论：氧自由基（ＯＦＲ）是ＩＡＲＦ的主要发生机制之一，ＩＡＲＦ确实存在着细胞凋亡的病理改变。ＭＥＬ预防用药能减轻ＩＡＲＦ，减少细胞凋亡的发生。

Ischemic Acute Renal Failure and Apoptosis

Objective:To investigate the relationship between ischemic acute renal failure (IARF) and the apoptosis,and the role of melatonin preventing IARF Methods:The animal model of IARF was set up by the operation of bilateral renal pedicle occlusion for 45 minutes and later reperfusion for 24 hours on SD rats The blood urea nitrogen(BUN),creatine(Cr),superoxide dismutase(SOD),glutathion peroxidase(GSH Px) and malondialdehyde(MDA)were examined for investigating the role of MEL preventing IARF Apoptosis was morphologically confirmed by in situ end labelling method of fragmented DNA using terminal dexoytransferase Results:the BUN,Cr of rats with IARF were increased,SOD and GSH Px were decreaced and MDA was higher A number of the renal tuberlar cells underwent apoptosis MEL can reverse the above changes,prevent or reduce the apoptosis at different levels depending upon the doses of MEL pre administered Conclusions:1 The renal tuberlar cells indeed undergo apoptosis in IARF 2 Preventive administering MEL can relieve IARF The effect is dose dependent