气相色谱法测定止痒酊中冰片薄荷脑的含量

目的:建立气相色谱法测定止痒酊中冰片、薄荷脑的含量.方法:采用石英毛细管柱(Agilent 0.5 3mm×1 5m),FID检测器,程序升温,初始温度80℃保持2 min后开始升温,以7C/min升温至140℃,分流进样,分流比为5∶1,进样量为1μL.结果:冰片质量浓度的线性范围0.5017～1.5051 mg/mL,平均回收率(n=6) 99.57％( RSD=0.90％)；薄荷脑质量浓度的线性范围0.4994～ 1.4982mg/mL,平均回收率(n=6)为99.55％ (RSD=1.03％).结论:气相色谱法灵敏、准确、可靠,可作为止痒酊质量控制方法.

Determination of borneolum syntheticum and menthol in Zhiyang Tincture by GC

Objective：To determine the contents of borneolum syntheticum and menthol in Zhiyang Tincture by GC.Methods：The analytical column was capillary column Agilent DB-WAX（0.53 mm ×15 m） with FID as detector.The column temperature was initially kept at 80℃,then gradually increased to140℃ with a rate of 7℃/min.The split injection was used,the split ratio was 5：1,and the injection volume was 1 μL.Results：The linear ranges of borneolum syntheticum and menthol were 0.5017～1.5051 and 0.4994～1.4982 mg/mL,respectively.And the average recoveries（n=6） were 99.57%（RSD=0.90%） and 99.55%（RSD=1.03%）,respectively.Conclusion：The method is simple,accurate and reliable,which can be used for the quality control of Zhiyang Tincture.