| 酵母菌药用乳糖酶制备新方法 |
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| 引用本文： | 酵母菌药用乳糖酶制备新方法[J]. 中国药科大学学报, 2004, (1): 88-91. |
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| 作者姓名： | 谭树华 高捷 海迪勒 尚新燕 高向东 吴梧桐 |
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| 作者单位： | 中国药科大学生命科学与技术学院,南京,210009 |
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| 摘 要： | 目的:建立一种简易的从酵母菌制备克鲁维酵母乳糖酶的方法.方法:在30L发酵罐中进行发酵,用对羟基苯甲酸酯进行细胞破壁,用超微过滤方法浓缩破壁液.结果:乳糖酶的收率可达到每毫升发酵液含11.4 ONPG单位.从酵终细胞中释放乳糖酶可达70%,每毫升牛奶中加入1.2 ONPG单位制备的液态乳糖酶可使牛奶中的乳糖水解率达到70%.
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| 关 键 词： | 脆壁克鲁维酵母 乳糖酶 破壁 对羟基苯甲酸酯 |
| 文章编号： | 1000-5048(2004)01-0086-04 |
| 修稿时间： | 2003-06-21 |
A Novel Method for Preparation of Lactase From Kluyeromyces Fragilis |
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| A Novel Method for Preparation of Lactase From Kluyeromyces Fragilis[J]. Journal of China Pharmaceutical University, 2004, (1): 88-91. |
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| Authors: | TAN Shu-Hu GAO Jie Hadeel A malek A Maji SHANG Xin-Yan GA O Xiang-Dong WU Wu-Tong |
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| Abstract: | AIM:To develop a simple method for prepare liquid lactase from kluyeronyces fragilis .METHOD:Fermentation was carried out in 30 L fermentor. The cell was disrupted by low concentration of p-hydroxybenzoate. The disrupted solution was concentrated by ultra-micro-filtration.RESULT:The lactase yield can reach 11.4 ONPG unit per 1 ml fermentatio n liquid. The lactase released from the yeast cell with the recovery of more th an 70%. The hydrolysis rate of lactose in milk can reach 70% by addition of the liquid lactase of 1.2 ONPG unit per 1 ml milk. |
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| Keywords: | Kluyeromyces fragilis% Lactase Propyl p-h ydroxybenzoate Cell disruption |
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