芍药苷对血管紧张素转化酶活性的抑制作用

目的：采用反相高效液相色谱法（RP-HPLC）测定了芍药苷对血管紧张素转化酶活性的影响。方法：采用Brava BDS C18色谱柱（4.6mm×250mm，5μm），流动相为乙腈-0.01％甲酸水溶液（25：75），柱温为30℃，流速为1.0mL·min^-1，检测坡长为228nm。结果：马尿酸在1.17-149μg·mL^-1（r=0.9999）范围内呈良好的线性关系；平均回收率（n=6）为100.4％（RSD=0.88％）；日内、日间精密度的RSD分别为1.32％和1.96％。芍药苷的IC50值为37.47μmol·L^-1。结论：该法分离效果和重复性均良好，结果准确可靠，可用于马尿酸的检测，也为血管紧张素转化酶抑制剂活性测定提供了可靠的方法。

Inhibitory Effects of Paeoniflorin on ACE Activity In Vitro

This is a study attempting to develop an efficient RP-HPLC method for determining the inhibitory activity of angiotensin converting enzyme （ACE）. The content of enzymolysis product hippuric acid was determined using RP - HPLC method. RP-HPLC was performed on a Brava BDS C18 column （4.6mm×250mm,5μm） using a mobile phase consisting of 0.01% formic acid solution-acetonitrile （25：75） at 30℃ with a flow rate of 1.0 mL·min^-1 and a detection wavelength at 228 nm. Test results showed that the calibration curve was hnear in the range of 1.17-149μg·mL^-1（r=0.9999） for hippuric acid. The average recovery （n=6） of hippuric acid was 100.4% （RSD=0.88%）. The intra-day and inter-day RSDs were at 1.32% and 1.96% respectively. The IC50 of Paeoniflorin was 37.47μg·mL^-1. Enjoying a sensitive, accurate, and reliable performance with good reproducibility, the method is desirable for determining the inhibitory activity of ACE.