卵巢癌患者外周血Th1/Th2细胞因子的变化趋势及其临床意义

目的:分析卵巢癌患者外周血Thl和Th2细胞的细胞因子变化,为其免疫治疗提供实验依据。方法:用刺激物刺激43例卵巢癌患者外周血CD3+T细胞增加细胞因子的表达,然后加入荧光标记的特异性抗细胞因子单克隆抗体,特异性抗体与抗原结合,最后应用流式细胞仪分析特异性细胞因子表达水平。用酶联免疫吸附法(ELISA)检测血清中相应的细胞因子水平,同时设正常对照组,以评价其水平变化趋势。结果:卵巢癌患者Thl型细胞因子干扰素-γ(IFN-γ)、白细胞介素-2(IL-2)、白细胞介素-12(IL-12)表达水平较正常对照组显著降低,Th2型细胞因子白细胞介素-4(IL-4)和白细胞介素-10(IL-10)表达水平较正常对照组升高,差异有统计学意义。肿瘤坏死因子-α(TNF-α)表达水平较正常对照组升高,差异有显著性。结论:卵巢癌患者Th2型细胞因子模式占优势,这可能是肿瘤细胞发生免疫逃逸,从而导致肿瘤的发生或者转移的原因之一。

Clinical significance of TH1/TH2 cytokines in the peripheral blood lymphocytes of patients with ovarian cancer

Objective:To analyzed level of Th1 and Th2 type cytokines in peripheral plasma of ovarian cancer.In order to find out the laboratory evidence of tumor immunotherapy.Methods:The peripheral blood lymphocytes in 43 cases of ovarian cancer were stimulated by stimulator in order to enhance expression of cytokines.Specific fluorochrome-conjugated monoclonal antibody combined with antigen.Serum cytokines were detected by flow cytometry and ELISA to evaluate the change tendency.We made normal controls.Results:The level of Th1 type cytokines such as interferon-γ(IFN-γ),interleukin-2(IL-2),interleukin-12(IL-12)were significantly lower in patients of ovarian cancer than those of normal controls.The level of Th2 type cytokines such as interleukin-4(IL-4),interleukin-10(IL-10)was higher in patients of ovarian cancer than those of normal controls,there were significant difference between them.The level of tumor necrosis factor a(TNF-a)in patients were significant higher than normal control.Conclusion:Th1/Th2 was imbalance in ovarian cancer.Th1 type cytokines were inhibited,and Th2 type cytokines were relatively enhanced,so Th1/Th2 shift to Th2.It maybe the mechanism of tumor arising and transferring by immune escaped from immunosurveillance.