| 荧光定量聚合酶链反应技术在胃幽门螺杆菌检测中的应用 |
| |
| 引用本文： | 薛进,袁建芬,喻海忠. 荧光定量聚合酶链反应技术在胃幽门螺杆菌检测中的应用[J]. 现代肿瘤医学, 2006, 14(9): 1110-1111 |
| |
| 作者姓名： | 薛进 袁建芬 喻海忠 |
| |
| 作者单位： | 1. 江苏省南通市肿瘤医院,江苏,通南,226361
2. 江苏省南通市中医院,江苏,南通,226001 |
| |
| 摘 要： | 目的:评估荧光定量PCR在检测胃粘膜上幽门螺杆菌的DNA中的应用价值。方法:用荧光定量PCR与普通PCR检测胃粘膜上的幽门螺杆菌的DNA,并对二者进行方法学比较。结果:荧光定量PCR阳性率为95.3%(143/150),普通PCR阳性率仅为86.0%(129/150),二者之间有明显的差异(P<0.01)。结论:荧光定量PCR在临床上检测幽门螺杆菌DNA方面具有简单、快速、敏感、特异的特点,在临床上具有较高的应用价值。
|
| 关 键 词： | 门螺杆菌 聚合酶链反应 DNA |
| 文章编号： | 1672-4992-（2006）09-1110-03 |
| 收稿时间： | 2006-03-16 |
| 修稿时间： | 2006-03-16 |
Detecting HP DNA in human gastric mucosa by FQ- PCR |
| |
| XUE Jin,YUAN Jian-fen,YU Hai-zhong. Detecting HP DNA in human gastric mucosa by FQ- PCR[J]. Journal of Modern Oncology, 2006, 14(9): 1110-1111 |
| |
| Authors: | XUE Jin YUAN Jian-fen YU Hai-zhong |
| |
| Abstract: | Objective:To evaluate the clinical application value of FQ-PCR(fluorogenic quantitative polymerase chain reaction) for detecting HP DNA in human gastric mucosa. Methods: HP-DNA in human gastric mucosa were detecting with FQ-PCR. Compare HP-DNA between FQ-PCR with PCR. Results: The postivity rate of FQ-PCR was 95.3%(143/150); the postivity rate of PCR was 86.0%(129/150), there was significant difference in measuring HP-DNA between the two methods (P<0.01). Conclusion: The method of FQ-PCR was simple, rapid, Sensitive, specific, could be used for detecting HP DNA. |
| |
| Keywords: | helicobacter pylori polymerase chain reaction DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
