| 高效液相色谱法测定羚羊清肺颗粒中黄芩苷含量 |
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| 引用本文： | 程晓英,付凌燕.高效液相色谱法测定羚羊清肺颗粒中黄芩苷含量[J].中国药业,2010,19(16):36-37. |
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| 作者姓名： | 程晓英 付凌燕 |
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| 作者单位： | 吉林省吉林市食品药品检验所,吉林,吉林,132001 |
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| 摘 要： | 目的建立羚羊清肺颗粒中黄芩苷含量测定的高效液相色谱（HPLC）法。方法色谱柱为Agilent Technologies Zorbax Extend-C18柱（250mm×4.6mm,5μm）,流动相为甲醇-水-磷酸溶液（47∶53∶0.2）,流速0.8mL/min,检测波长280nm,柱温30℃。结果黄芩苷质量浓度在200.0～1000.0μg/mL范围内与峰面积线性关系良好,平均加样回收率为99.53%,RSD=0.28%（n=6）。结论该方法简便易行,准确可靠,可用于羚羊清肺颗粒的质量控制。
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| 关 键 词： | 高效液相色谱法 羚羊清肺颗粒 黄芩苷 含量测定 |
Determination of Baicalin Content in Lingyang Qingfei Granules by HPLC |
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| Cheng Xiaoying,Fu Lingyan.Determination of Baicalin Content in Lingyang Qingfei Granules by HPLC[J].China Pharmaceuticals,2010,19(16):36-37. |
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| Authors: | Cheng Xiaoying Fu Lingyan |
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| Institution: | ( Jilin Institute for Food and Drug Control, Jilin, Jilin, China 132001) |
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| Abstract: | Objective To set up a determination method of baicalin content in Lingyang Qingfei Granules. Methods The HPLC method was developed to quantitative determination. The Agilent Technologies Zorbax Extend-C18 column（250 mm × 4. 6 mm,5 μm） was used. The mobile phase was methanol-water-phosphoric acid（47 ∶ 53 ∶ 0. 2） . The column temperature was 30 ℃ ,the wavelength for detection was 280 nm,and the flow rate was 0. 8 mL / min. Results The linear relation was obtained in the range of 200. 0-1 000. 0 μg / mL for baicalin. The average recovery rate of baicalin was 99. 53% ,and RSD was 0. 28% （ n = 6） . Conclusion The method is simple,accurate,reliable and suitable for the quality control of Lingyang Qingfei Granules. |
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| Keywords: | HPLC Lingyang Qingfei Granules baicalin content determination |
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