心房颤动犬心房组织炎症因子的表达及意义

目的 探讨炎症因子白细胞介素-1β(IL-1β)、C-反应蛋白(CRP)在心房颤动(房颤)时的表达及意义.方法 健康杂种犬16条,随机分为对照组(n=8)和房颤组(n=8).房颤组置入永久起搏器,起搏时间24周.制备房颤犬模型,对照组不置入起搏器.在左、右心房切取组织块,甲醛固定后石蜡包埋保存.两组石蜡切片均行免疫组织化学检测IL-1β和CRP,比较两组间IL-1β、CRP的表达水平.以平均吸光度表示各组IL-1β、CRP的表达水平,进行半定量分析.结果 房颤组IL-1β及CRP的平均吸光度分别为(13.8±3.5)%、(13.4±2.8)%,均明显高于对照组[(11.1±3.8)%,(12.0±3.0)%],均P<0.05).结论 炎症因子IL-1β和CRP可能在房颤的发生和维持中起着促进的作用.

Study on expression and significance of inflammatory factors in dog atria with atrial fibrillation

Objective To investigate the expression and significance of interleukin-1β and C-reactive pro-tein(CRP)in atrial fibrillation(AF).Methods Sixteen mongrel dogs were randomly divided into two groups:pa-cing group(n=8)and control group(n=8).High frequency pacing was performed in the pacing group with perma-nent pacernaker but not in the control group.After pacing for 24 weeks,tissue samples were obtained from the left and right atria.The levels of IL-1βand CRP in atria were detected by immunohistochemistry.Results Compared to the control group,the expression level of IL-1β and CRP were significantly enhanced(P<0.05)in atrial tissues during AF.Conclusion IL-1β and CRP may participate in atrial fibrillation formation and play a role in generation and maintaining the artrial fibrillation.