电针对内毒素休克大鼠肝脏的影响(组织化学观察) |
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引用本文: | 黄为敏 黄坤厚. 电针对内毒素休克大鼠肝脏的影响(组织化学观察)[J]. 针刺研究, 1995, 20(3): 36-39 |
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作者姓名: | 黄为敏 黄坤厚 |
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作者单位: | 中国中医研究院针灸所!北京100700 |
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摘 要: | 本研究以组化法观察电针对内毒素休克鼠肝脏的影响。结果表明内毒素休克鼠肝糖元几乎全部脱失,葡萄糖-6-磷酸酶(G-6-Pase),琥珀酸脱氨酶(SDH),5’-核苷酸酶(5’-Nase),镁激活的三磷酸腺苷(Mg++-ATPase)活性减弱,电针后糖元含量部分增加,SDH、Mg++-ATPase和G-6-Pase活性较休克对照组增加。提示电计“人中”或“足三里”穴可能对改善肝脏代谢和提高膜的转运起一定的作用。
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关 键 词: | 内毒素休克 电针“人中” 组织化学 肝脏 肝糖元 酶 |
Histochemical Observation of the Effect of Electroacupuncture on the Livers of Rats with Endotoxic Shock |
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Abstract: | The expcrimental rats were randomly divided into three groups: L e. controI group; endotoxicshock group (the model of endotoxic shock was induced by intravenous administration of E. Coli endotoxin, 16mg/kg ): and electroacupuncture (EA ) group (" Renzhong" or ' Zusanli)' acupolnt wasstirnulated for 1o minutes at l hr after injection of endotoxin)- The experimental rats were decapitated at 75 minutes after injccting endotoxin. Their livers were taken out for cryostat section - and histo-chemical observation. The results were as follows: 1 ) The glycogen in the hepatic cells of endotoxicshocked rats were alrnost completely depleted- The activities of SDH Mg++u ATPase. G-6-Pase and5'~Nase were decreased: especially the activities of 5'-Nase 1n the biliary canallcull and sinusoids wereapparently rec1uced. 2 ) The content of hepatic glycogen in EA group was increased, but some of themwas still dePleted. The activities of SDH, Mg++-ATPase and G-6-Pase were slightly increased ascompared wlth that of the endotoxic shock group. The activity of 5'uNase was obviously increased afte r EA. The prelin1inary results indicated that EA at "Renzhong" or IlZusanliI' acupoint of rats withndotoxic shock mlght play certain role on lmproving the hepatic metabolism and prornoting the rnembrane transport action |
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