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| 肝素联合肝干细胞经脾移植治疗SD大鼠急性肝损伤 | |
| 作者姓名： | Huang Z Zeng S Ouyang M Dong J Gong Y Shen H |
| 作者单位： | 1.中南大学湘雅医院医学实验研究中心,长沙 410008; 2.中南大学湘雅医院肿瘤科,长沙 410008; 3.中南大学湘雅医院消化内科,长沙 410008; 4.加拿大Manitoba大学药学院,Winnipeg MB R3E 0T5, Canada |
| 基金项目： | 国家自然科学基金,湖南省中医药管理局科研基金 |
| 摘 要： | 目的:探讨肝素联合肝干细胞(WB-F344细胞)经脾移植对大鼠急性肝损伤的治疗作用.方法:构建携带绿色荧光蛋白(GFP)基因的慢病毒载体,体外培养、扩增WB-F344细胞,携带GFP基因的慢病毒转染WB-F344细胞.通过腹腔注射四氯化碳(CCl4)建立大鼠急性肝损伤模型,造模后24 h分别将1 mL含2×107个肝干... |
| 关 键 词： | 肝素 肝干细胞 经脾移植 急性肝损伤 |
Treatment of acute liver injury by intrasplenic transplantation of hepatic stem cells combined with heparin in rats |
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| Huang Z,Zeng S,Ouyang M,Dong J,Gong Y,Shen H.Treatment of acute liver injury by intrasplenic transplantation of hepatic stem cells combined with heparin in rats[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),2011,36(5):411-416. | |
| Authors: | Huang Zenghui Zeng Shan Ouyang Miao Dong Juanjuan Gong Yuewen Shen Hong |
| Institution: | 1.Medical Research Center, Xiangya Hospital, Central South University, Changsha 410008, China; 2.Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, China; 3.Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410008, China; 4.Faculty of Pharmacy, University of Manitoba, Winnipeg MB R3E 0T5, Canada |
| Abstract: | ObjectiveTo determine the treatment effects of transplanted hepatic progenitor cells (WB-F344 cells) combined with heparin on the acute liver injury in SD rats. MethodsA total of 2×107 hepatic stem cells (WB-F344) infected with GFP lentivirus and 8 μL heparin were transplanted through the spleen in SD rats with acute liver injury, which was induced by an intraperitoneal injection of CCl4. The liver and spleen tissues underwent fluorescence examination 1 day after the transplantation. The liver functions were tested, and the liver tissues were histopathologically examined on the 3rd, 7th, 14th, and 28th day of the cell transplantation. ResultsThe transfected WB-F344 cells expressed GFP 3 days after the lentivirus infection and were found in the rat liver 1 day after the WB-F344 transplantation. The liver function and histopathological recovery of the liver tissues in the group of WB-F344 transplantation were better than those of the control group (P<0.05).ConclusionTransplantation of hepatic stem cells combined with heparin can promote the liver recovery in rats with acute liver injury induced by CCl4. |
| Keywords: | heparin hepatic stem cells intrasplenic transplantation acute liver injury |
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