| X线照射对人结直肠癌细胞株SW480中Kiss-1基因表达的影响 |
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| 引用本文： | 涂明美,陈绍勤,戴起宝,柯春林,林素勇.X线照射对人结直肠癌细胞株SW480中Kiss-1基因表达的影响[J].肿瘤基础与临床,2011,24(2):95-97. |
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| 作者姓名： | 涂明美 陈绍勤 戴起宝 柯春林 林素勇 |
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| 作者单位： | 1. 福建医科大学附属第一医院胃肠外科,福建,福州,350005
2. 福建医科大学附属第一医院放疗科,福建,福州,350005 |
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| 基金项目： | 福建省自然科学基金项目，福建省卫生厅青年科研课题项目 |
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| 摘 要： | 目的探讨X线照射对体外培养人结直肠癌细胞株SW480中肿瘤转移抑制基因Kiss-1表达的影响。方法应用SYBRGreen实时定量PCR检测、相对定量法分析不同剂量X线照射后24、48 h SW480中Kiss-1 mRNA的表达水平。结果与对照组相比,照射后24 h,各照射组Kiss-1 mRNA表达的差异均无统计学意义(P>0.05);照射后48 h,2、4、6 Gy组Kiss-1 mRNA表达上调(P<0.05),8 Gy组Kiss-1 mRNA的表达差异无统计学意义(P>0.05)。结论 X线照射不会导致结直肠癌细胞Kiss-1基因表达下调,放疗可能不会增加结直肠癌转移的风险。
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| 关 键 词： | 结直肠肿瘤 电离辐射 肿瘤转移 Kiss-1 聚合酶链反应 |
The Influence of X-Ray Radiation on Expressions of Kiss-1 Gene in Human Colorectal Cancer Cell Line SW480 |
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| Tu Mingmei,Chen Shaoqin,Dai Qibao,Ke Chunlin,Lin Suyong.The Influence of X-Ray Radiation on Expressions of Kiss-1 Gene in Human Colorectal Cancer Cell Line SW480[J].journal of basic and clinical oncology,2011,24(2):95-97. |
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| Authors: | Tu Mingmei Chen Shaoqin Dai Qibao Ke Chunlin Lin Suyong |
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| Institution: | 1(1.Department of Gastrointestinal Surgery,the First Affiliated Hospital of Fujian Medical University;2.Department of Radiotherapy,Fuzhou 350005,China) |
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| Abstract: | Objective To investigate the influence of X-ray radiation on Kiss-1 gene expressions of colorectal cancer cell line SW480 in vitro.Methods SW480 was irradiated at different doses.The expression differences of Kiss-1 mRNA were detected using SYBR Green quantitative real-time PCR at 24,48 h.Then the relative quantification method was used to analyze the differences.Results For the Kiss-1 gene expressions of SW480,there was no significant difference 24 h after radiation(P>0.05).However,after radiation with 2,4,6 Gy for 48 h,the Kiss-1 mRNA expressions were increased(P<0.05),but there was no significant difference for 8 Gy group(P>0.05).Conclusion Radiation can′t decrease Kiss-1 gene expressions of colorectal cancer cells,and the radiotherapy can′t increase the risk of colorectal cancer metastases. |
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| Keywords: | Kiss-1 |
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