| ET-1及其受体ETAR在食管癌中的表达及意义 |
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| 引用本文： | 矫文捷,王天佑,丁芳,孔建平,刘芝华,潘浩. ET-1及其受体ETAR在食管癌中的表达及意义[J]. 第三军医大学学报, 2005, 27(23): 2314-2316 |
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| 作者姓名： | 矫文捷 王天佑 丁芳 孔建平 刘芝华 潘浩 |
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| 作者单位： | 北京大学第一医院胸外科,北京,100034;首都医科大学附属北京友谊医院胸外科,北京,100050;中国医学科学院肿瘤研究所分子肿瘤学国家重点实验室,北京,100021 |
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| 摘 要： | 目的研究内皮素-1(endothelin-1,ET-1)及其受体ETAR和食管癌生长转移的关系.方法采用RT-PCR及Western blot检测观察食管癌及配对癌旁食管黏膜组织中的ET-1和ETAR的表达,并结合临床病理特征分析其意义.结果ET-1 mRNA在食管癌组织中的阳性表达率为82.7%(62/75),在癌旁食管黏膜组织的阳性表达率为60%(45/75),且其在癌组织中的过度表达与肿瘤分化程度、淋巴结转移、临床分期有关系(P＜0.05);ETAR mRNA在食管癌组织中的阳性表达率为61.3%(46/75),在癌旁食管黏膜组织的阳性表达率为62.7%(47/75),两者比较无显著性差异(P＞0.05).另外,Westernblot检测显示在74.7%(56/75)的食管癌组织中有ET-1蛋白高表达.结论ET-1与食管癌生长转移有密切关系.
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| 关 键 词： | 食管肿瘤 内皮素1 逆转录多聚酶链式反应 |
| 文章编号： | 1000-5404(2005)23-2314-03 |
| 收稿时间： | 2005-08-16 |
| 修稿时间： | 2005-09-15 |
Expression and clinical significance of endothelin-1 and endothelin type A receptor gene in esophageal cancer |
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| JIAO Wen-jie,WANG Tian-you,DING Fang,KONG Jian-ping,LIU Zhi-hua,PAN Hao. Expression and clinical significance of endothelin-1 and endothelin type A receptor gene in esophageal cancer[J]. Acta Academiae Medicinae Militaris Tertiae, 2005, 27(23): 2314-2316 |
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| Authors: | JIAO Wen-jie WANG Tian-you DING Fang KONG Jian-ping LIU Zhi-hua PAN Hao |
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| Affiliation: | 1. Department of Thoracic Surgery, The First Hospital of Peking University, Beijing 100034; 2.Department of Thoracic Surgery, Beijing Friendship Hospital Affiliate of Capital University of Medical Sciences, Beijing 100050; 3. Department of Molecular Ontology, Tumor Institute, Chinese Academy of Medical Sciences, Beijing 100021, China |
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| Abstract: | Objective To explore the relationship between the ET-1 and the oncogenesis, development of human esophageal cancer. Methods RT-PCR and Western blotting were employed to detect the expression of ET-1 and ETAR in the tissues of esophageal cancer and normal esophageal mucous membrane from 76 esophageal cancer patients admitted by our hospital from March to December in 2002. Results There was positive ET-1 mRNA expression in 82.7% (62/75) of the specimens of esophageal cancer and in 60% (45/75) of normal tissue and the ET-1 mRNA expression had a relation with histological grade, lymph node metastasis and clinical staging of esophageal cancer (P<0.05). The positive ETAR mRNA expression was found in 61.3% (46/75) of the specimens of esophageal cancer and in 62.7% (47/75) of normal tissue (P<0.05). Western blotting showed that 56 of 75 specimens of esophageal cancer had an overexpression of ET-1 protein. Conclusion ET-1 may play an important role in the oncogenesis, development of esophageal cancer. |
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