乙肝病毒preS1基因与截短C基因真核表达载体构建及表达

目的建立乙型肝炎病毒（HCV）preS1基因与截短C基因真核表达载体，并在CHO细胞中瞬时表达。方法PCR方法扩增HBV核心区羧基端部分缺失的基因片段和preS1全基因，克隆入真核表达载体pcDNA3．1（-）后测序鉴定，并通过脂质体瞬时转染CHO细胞。结果构建了真核表达载体pcDNA3．1（-）-Ct-preS1，成功转染CHO细胞，间接免疫荧光和RT—PCR证实pcDNA3．1（-）-Ct-preS1在CHO中表达截短的核心基因和preS1基因融合蛋白。结论成功构建preS1基因与截短C基因真核表达载体，可在CHO细胞中瞬时表达，为深入研究HBV基因免疫奠定了基础。

Construction of Expression Vector of Truncated C Gene Combined With preS1 Gene of Hepatitis B Virus and Expression in Eukaryotic Cell

Objective To construct the eukaryotic expressing vector of truncated C gene combined with preS1 gene of hepatitis B virus and transiently expressed in CHO cell line. Methods The sequences encoding truncated HBV core gene which lack parts of the carboxyl-terminal and preS1 gene were amplified by PCR before inserted into plasmid pcDNA3.1(-),then recombined plasmid pcDNA3.1(-)-Ct-preS1 was transiently transfected CHO by liposome. Results Eukaryotic expression vector was constructed and transfected into CHO cell line transiently. Expected fused protein was expressed in CHO cell and confirmed by IFA and RT-PCR. Conclusion Successful construction of eukaryotic expression vector of truncated C gene combined with preS1 gene is obtained. It could transiently express in CHO cell line and establish basis for further study on HBV.