A Method to Determine Significant Levels of Immunoglobulin G to Aspergillus Fumigatus Antigens in an ELISA System and a Comparison with Counterimmunoelectrophoresis and Double Diffusion Techniques |
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| Authors: | Veronica M. Hearn G. C. Donaldson M. J. R. Healy D. M. Trotman |
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| Affiliation: | 1. London School of Hygiene and Tropical Medicine Mycological Reference Laboratory , London, WCIE 7HT, England;2. London School of Hygiene and Tropical Medicine Mycological Reference Laboratory , London, WCIE 7HT, England;3. Department of Medical Statistics , Keppel St. , London, WCIE 7HT, England;4. MRC Pneumoconiosis Unit, Llandough Hospital Penarth , S. Glamorgan, CF6 1XW, Wales, U.K. |
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| Abstract: | A method is described which assesses results obtained from an ELISA system for the determination of human serum levels of IgG class antibodies to Asperglllus fumigatus. The method is used to discriminate positive from negative samples, and significant antibody activity may be reported to the clinician, relative to a reference positive control serum monitored simultaneously under the same test conditions. Antibody content is expressed as the absorbance of a certain dilution of serum. Duplicate samples were analysed at a single serum dilution and their absorbtion values obtained from a semi-automated ELISA microplate reader. These were entered into a computer programmed to convert the data into units on a logarithmic scale. In parallel experiments, ELISA results were compared with those obtained by the techniques of counterimmunoelectrophoresis and double diffusion which measure precipitating antibody of all classes. A relatively good degree of correlation between tests was found only among sera with a high level of antibody. |
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