Reanalysis of Antisera Specificities and Binding Characteristics of Rat Pituitary Hormone Assays |
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| Authors: | Kerry L Cheesman Robert T Chatterton Jr Craig W Beattie Carl B Wallemark Walter W Hauck |
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| Institution: | 1. Northwestern University Medical School, Dept. of Obstetrics and Gynecology Chicago , Illinois, 60611;2. University of Illinois at the Medical Center Div. of Surgical Oncology, Dept. of Surgery Chicago , Illinois, 60612;3. Northwestern University Medical School Biometry Section, Cancer Center Chicago , Illinois, 60611 |
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| Abstract: | Rat pituitary hormone radioimmunoassays (RIAs) are widely used in reproductive research, yet data on specificity and binding characteristics of many of the antisera are not widely available. This report characterizes one set of rat antisera supplied by the National Institutes of Health (USA). Rat follicle-stimulating hormone (FSH) and thyrotropin-stimulating hormone (TSH) antisera appear specific, but TSH exhibited significant competition in the rat luteinizing hormone (LH) assay. In addition, statistically significant nonparallelism was demonstrable in all three assay systems. This creates further problems in characterizing antisera cross-reactivity and may make potency estimates for pituitary standards inaccurate. |
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| Keywords: | Rat Pituitary Radioimmuno-assay |
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