| 核酸外切酶-压电石英DNA传感器检测金黄色葡萄球菌的实验研究 |
| |
| 引用本文： | 黄君富,夏涵,黄庆,府伟灵.核酸外切酶-压电石英DNA传感器检测金黄色葡萄球菌的实验研究[J].第三军医大学学报,2009,31(13). |
| |
| 作者姓名： | 黄君富 夏涵 黄庆 府伟灵 |
| |
| 作者单位： | 第三军医大学西南医院检验科,重庆,400038 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金 |
| |
| 摘 要： | 目的 构建一种用于检测金黄色葡萄球菌的核酸外切酶-压电石英DNA传感器新方法,同时对其重要影响因素进行探讨.方法 采用λ核酸外切酶处理金黄色葡萄球菌PCR产物,EB染色、电泳后于紫外灯下进行观察,对杂交温度、杂交时间和杂交响应性能等因素进行实验研究,进一步将构建的核酸外切酶-压电石英DNA传感器法用于金黄色葡萄球菌检测,并对其灵敏度和特异性进行研究.结果 λ核酸外切酶-压电石英DNA传感器法最适杂交温度为35℃,最适杂交时间为60 min,响应性能显著大于普通压电DNA传感器(P＜0.01),可以检测到1.0×104CFU/ml的金黄色葡萄球菌,特异性好.结论 利用核酸外切酶-压电石英DNA传感器检测金黄色葡萄球菌,具有杂交效率高、技术难度低、频率响应性能高等优点,有较好的应用前景.
|
| 关 键 词： | 石英晶体微天平 生物传感器 λ核酸外切酶 金黄色葡萄球菌 |
Detection of Staphylococcus aureus by a lambda exonuclease based piezoelectric quartz crystal microbalance DNA sensor |
| |
| HUANG Jun-fu,XIA Han,HUANG Qing,FU Wei-ling.Detection of Staphylococcus aureus by a lambda exonuclease based piezoelectric quartz crystal microbalance DNA sensor[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(13). |
| |
| Authors: | HUANG Jun-fu XIA Han HUANG Qing FU Wei-ling |
| |
| Institution: | HUANG Jun-fu,XIA Han,HUANG Qing,FU Wei-ling (Department of Laboratory Medicine,Southwest Hospital,Third Military Medical University,Chongqing 400038,China) |
| |
| Abstract: | Objective To develop a new type of piezoelectric quartz crystal microbalance DNA sensor based on lambda exonuclease to detect Staphylococcus aureus (S. aureus) and optimize the main detection conditions. Methods After the DNA was extracted from the stain of S. aureus and the target fragment was amplified with self-designed universal primers, the PCR products were treated with Lambda exonuclease and visualized by agarose gel electrophoresis with ethidium bromide followed by a gel documentation system analysi... |
| |
| Keywords: | quartz crystal microbalance biosensor lambda exonuclease Staphylococcus aureus |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
