Amplification of genomic DNA fragments of Sarcocystis muris (Apicomplexa) cyst merozoites encoding a thiol (cysteine) proteinase |
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| Authors: | Thomas Hansner Bernd Freyer Heinz Mehlhorn Wolfgang Rüger |
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| Affiliation: | Ruhr Universit?t Bochum, Arbeitsgruppe Molekulare Genetik, D-44780 Bochum, Germany Tel.: +49-234 700-3102; Fax: +49-234 7094-195; e-mail: wolfgang.rueger@ruhr-uni-bochum.de, DE
Heinrich Heine Universit?t, Institut für Zoomorphologie, Zellbiologie und Parasitologie, D-40225 Düsseldorf, Germany, DE
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| Abstract: | Parasites of the phylum Apicomplexa (Sporozoa) cause diseases such as malaria, toxoplasmosis, or intestinal coccidiosis. Invasive stages possess typical apical organelles such as dense granules that harbor a broad range of polypeptides that are believed to take part in the parasite-host cell interaction. In previous studies a 26-kDa polypeptide of dense granules from Sarcocystis muris cyst merozoites (bradyzoites) was characterized as a thiol (cysteine) proteinase. In this paper a method is demonstrated to amplify DNA fragments from genomic DNA of S. muris cyst merozoites by polymerase chain reaction, which probably code for the 26-kDa antigen. Received: 12 December 1997 / Accepted: 17 January 1998 |
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