三化汤对脑缺血再灌注大鼠脑组织基质金属蛋白酶-9表达的影响

目的:观察三化汤对脑缺血再灌注大鼠脑组织基质金属蛋白酶-9(MMP-9),MMP-9 mRNA表达的影响.方法:将大鼠随机分为假手术组、模型组、三化汤低、高剂量组(7.2,14.4 g·kg-1) ig、尼莫地平组(8.1 mg·kg-1)ig.大鼠常规饲养3d后ig给药,每日1次,连续给药7d后采用线栓法制备脑缺血再灌注大鼠模型.采用免疫组织化学法检测各组大鼠脑组织MMP-9的表达,采用逆转录聚式酶链反应法(RT-PCR)检测脑组织MMP-9 mRNA的表达.结果:模型组脑组织MMP-9,MMP-9mRNA表达显著升高(P＜0.01)；三化汤高剂量组脑组织MMP-9,MMP-9 mRNA表达显著降低(P＜0.01),尼莫地平组脑组织MMP-9,MMP-9 mRNA表达也明显降低(JP＜0.05),三化汤低剂量组脑组织MMP-9,MMP-9 mRNA表达降低不明显；三化汤高剂量组降低脑组织MMP-9,MMP-9 mRNA表达较尼莫地平组明显.结论:三化汤对大鼠脑缺血再灌注损伤具有一定的保护作用.

The Effect of Sanhua Tang on Expression of MMP-9 of Brain Tissues in Cerebral Ischemia-reperfusion Rat

Objective: To observe the effect of Sanhua Tang on expression of MMP-9 in brain tissues of cerebral ischemia-reperfusion rat. Method: The rats were randomly allotted 5 groupa as following: sham operation group, model group, low dose group of Sanhua Tang(7.2 g·kg^-1),large dose group of Sanhua Tang(14.4 g·kg^-1)ig, and nimodipine group(8.1 mg·kg^-1)ig. After 3 days of routine breeding, Sanhua Tang was administered to rats by intragastric administration once every day for a week.The cerebral ischemia reperfusion model was reproduced by blocking middle cerebral arteryt with suture method. The expression of MMP-9 protein was detected by immunohistochemical methods with marketable kit, and mRNA expression of MMP-9 was assayed by RT-PCR. Result: In model group the expression of MMP-9 and MMP-9 mRNA in brain tissues was significantly increased(P<0.01).In large dose group of Sanhua Tang the expression of MMP-9 and MMP-9 mRNA was significantly decreased (P<0.01).In nimodipine group the expression of MMP-9 and MMP-9 mRNA was significantly decreased (P<0.05).In low dose group of Sanhua Tang the expression of MMP-9, and MMP-9 mRNA was less significantly decreased. In large dose group of Sanhua Tang the expression of MMP-9,MMP-9 mRNA was decreased more significantly than in nimodipine group. Conclusion: The mechanism of Sanhua Tang protecting against injure of cerebral ischemia reperfusion may be involved in ameliorating MMP expression.