氧化苦参碱对结肠癌LoVo细胞c-myc,PSMD9,CDK4mRNA表达的影响

目的:探讨氧化苦参碱( oxymatrine,OM)抑制人结肠癌LoVo细胞增殖和诱导凋亡的分子作用机制.方法:采用流式细胞仪检测LoVo细胞凋亡率以及细胞周期分布；采用荧光定量PCR法检测0.25,0.5 g·L-1 OM对LoVo细胞增殖相关基因c -myc,蛋白酶调解因子9(PSMD9),CDK4的基因表达的影响.结果:0.5 g·L-1以下浓度的OM作用结肠癌LoVo细胞48 h,对细胞凋亡无明显影响.0.25 g·L-1 OM作用48 h时可明显抑制人结肠癌LoVo细胞c-myc基因表达(P＜0.05).0.5g·L-1 OM作用48 h时可明显抑制LoVo细胞c-myc,CDK4的基因表达(P ＜0.01,P＜0.01,).药物作用时间为96 h时,0.5g·L-1 OM可明显抑制c-myc,PSM D9,CDK4基因表达(P＜0.05,或P＜0.01).结论:较低剂量OM显著抑制人结肠癌LoVo细胞增殖的作用机制,可能与下调LoVo细胞c-myc,PSM D9,CDK4表达有关.

Effect of Oxymatrine on Expression of c-myc, PSMD9 and CDK4 mRNA in Human Colon Carcinoma LoVo Cells

Objective: To explore the molecular mechanism of inhibiting colon cancer cell strein LoVo proliferation and inducing apoptosis by oxymatrine (OM) Method: Flow cytometry was used to detect the LoVo cells apoptosis and cell cycle distribution. Fluorescence quantitative PCR was used to detect cell proliferation-related genes like the c-myc, proteasome modulator 9(PSMD9), CDK4 gene expression when LoVo was treated with 0.25,0.5 g·L^-1OM. Result: OM had no significant effect on apoptosis in colon cancer LoVo cells when the treatment of OM lasted 48 h and the concentration was lower than 0.5, 0.25 g·L^-1 OM can inhibit c-myc gene expression in LoVo when duration of action last 24 h (P<0.05). When the dose increated to 0.5 g·L^-1 and duration of action was 48 h, OM could inhibit c-myc, CDK4 gene expression in LoVo cells (P<0.01, P<0.01). When duration of action was extended to 96 h, 0.5 g·L^-1 OM could inhibit the c-myc, PSMD9, CDK4 gene expression in LoVo cells(P<0.05,P<0.01, P<0.01). Conclusion: OM at Lower dose could significantly inhibit the proliferation of human colon cancer LoVo cells, the mechanism may be related to reducing c-myc, PSMD9, CDK4 expression in LoVo cells.