肉苁蓉对大鼠力竭游泳能力和心肌线粒体抗氧化能力的影响

目的:研究肉苁蓉对大鼠运动能力及心肌线粒体抗氧化能力的影响.方法:7周龄清洁级雄性Wistar大鼠120只随机分为静止ig水组(C组)、静止ig肉苁蓉组(M组),运动ig水组(T组)、运动ig肉苁蓉l～4组(TM1 ～4组).每天ig 1次.TM1 ～4组ig剂量依次为6.01,9.97,13.94,17.90 g·kg -1,ig体积为5 mL·kg -1,C,T2组ig等量蒸馏水.4周力竭游泳训练后,测定体重、力竭游泳时间及心肌线粒体中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)含量等与疲劳作用相关的指标.结果:TM各组体重大于T组(P＜0.05),力竭游泳时间均长于T组(P＜0.01),TM各组间无显著性差异.力竭游泳导致大鼠心肌线粒体中MDA含量显著升高,T组及TM 1 ～4组分别升高至(5.96±0.71),(5.05±0.36),(5.05 +0.92),(5.06±0.16),(5.05±0.25) μmol·L-1,且TM各组明显低于T组(P ＜0.01)；SOD,GSH-Px活性显著下降(T组及T1 ～T4组SOD分别下降至(209.7±817.21),( 249.85±10.93),(253.98±9.64),(259.311±4.57),(259.461±5.73)U·mL-1,GSH-Px分别下降至(57.95±11.95),(68.58±6.01),(70.63±5.83),(70.78±7.04),(72.56±4.34) U·mg-1,且TM各组明显高于T组(P＜0.0l)；TM各组间各项指标无显著性差异.结论:肉苁蓉具有提高大鼠运动能力和心肌线粒体抗氧化酶活性的作用,从而减轻自由基对心肌线粒体膜和肌浆网膜造成损伤,抑制大强度力竭运动造成的心肌线粒体氧化损伤,延缓疲劳发生；有显著作用的剂量为6.01 g.kg-1.

Effect of Cistanches Herba on the Swimming Ability and Oxidation Resistance of Mitochondrial in Rats

Objective:To study the effectd of Cistanche Herba on swimming ablity antioxidant resistance of.mitochondrial in rats Method:One hundred and twenty male Wistar rats were randomly divided into static ig water group(C group),still ig Cistancht Herba group(M group),exercise ig water group(T group),group exercise ig Cistanches Herba 1-4(TM 1-4 group).A professional device ig 1 times a day.TM 1-4 ig dose group were 6.01,9.97,13.94,17.90 g·kg-1,ig volume 5 mL·kg-1,C,T two groups ig distilled water.4 weeks after exhaustive swimming training,measured body weight,swimming time and heart mitochondria in malondialchehy-che(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) levels and other indicators related with fatigue.Result:TM weight of each group is greater than the T group(P<0.05),swimming time longer than T group(P<0.01),TM each group no significant differences.Result of exhaustive swimming in the MDA content in rat heart mitochondria was significantly increased,T-group and TM 1-4 group were increased to(5.96±0.71),(5.05±0.36),(5.05±0.92),(5.06±0.16),(5.05 ±0.25) μmol·L-1,and that the TM groups were significantly different lower than the T group(P<0.01);SOD,GSH-Px activity was significantly decreased(T-group and T1-T4 group,SOD decreased to(209.78±17.21),(249.85±10.93),(253.98±9.64),(259.31±14.57),(259.46±15.73) U·mL-1,GSH-Px decreased to(57.95±11.95),(68.58±6.01),(70.63±5.83),(70.78±7.04),(72.56±4.34) U·mg-1,and that the TM groups were significantly higher than the T group(P<0.01);TM of each group showed no significant difference.Conclusion:Cistanches Herba has improved exercise capacity in rat heart mitochondria and the role of antioxidant enzymes,there by reducing free radicals on myocardial sarcoplasmic reticulum and mitochondrial membrane damage,inhibition of high-intensity exhaustive exercise cause myocardial mitochondrial oxidative damage,delay fatigue occurred;have a significant effect at a dose of 6.01 g·kg-1.