蛇床子-补骨脂在乳腺癌骨转移裸鼠模型中吸收入血特征的研究

目的:以蛇床子素和补骨脂素为靶标成分,从药物吸收入血角度探讨蛇床子-补骨脂配伍后疗效增加的机制。方法:采用左心室注射MDA-MB-231BO细胞法建立裸鼠乳腺癌骨转移模型,给予不同配比的蛇床子-补骨脂药物煎液,采用HPLC-MS法测定裸鼠血浆中蛇床子素和补骨脂素,观察蛇床子-补骨脂配伍后蛇床子素和补骨脂素吸收入血的特征。结果:蛇床子组血浆中可发现蛇床子素离子峰;补骨脂组血浆中可发现补骨脂素分子及其离子峰信号;蛇床子-补骨脂(1:3)组血浆中可发现蛇床子素分子及离子峰信号,未发现补骨脂素分子及离子峰信号,蛇床子-补骨脂(2:2)组可发现蛇床子素和补骨脂素的分子及离子峰信号,蛇床子-补骨脂(3:1)组可发现蛇床子素分子及离子峰信号,未发现补骨脂素分子及离子峰信号。结论:蛇床子素和补骨脂素均可被吸收入血;蛇床子与补骨脂1:1配伍可促进补骨脂中补骨脂素的吸收入血。

Study on Characteristics of Absorption into Blood of Common Cnidium Fruit-Malaytea Scurfpea Fruit in Breast Cancer Bone Metastasis Nude Mice Model

Objective: To investigate the mechanism of curative effect increased of Common Cnidium Fruit-Malaytea Scurfpea Fruit compatibility based on osthole and psoralen as the target components absorbed into the bloodstream. Method: The breast cancer bone metastasis nude mice models were established by injecting MDA-MB-231BO cells into left ventricular, and they were given different combinations of Common Cnidium Fruit-Malaytea Scurfpea Fruit drugs decoction, osthole and psoralen in nude ice plasma were detected by HPLC-MS, and the characteristics of osthole and psoralen absorbed into the bloodstream were observed. Result: The plasma osthole's ion peak could be found in the plasmas of Common Cnidium Fruit group; the psoralen molecule and its ion peak signal could be found in the plasmas of Malaytea Scurfpea Fruit group; osthole molecule and its ion peak signal, but not psoralen molecule or its ion peak signal could be found in the plasmas of Common Cnidium Fruit-Malaytea Scurfpea Fruit (1: 3) group; not only osthole molecule and its lon peak signal but also psoralen molecule and its lon peak signal could be found in the plasmas of Common Cnidium Fruit-Malaytea Scurfpea Fruit (2: 2) group; osthole molecule and its ion peak signal, but not psoralen molecule or its ion peak signal could be found in the plasmas of Common Cnidium Fruit-Malaytea Scurfpea Fruit (3: 1) group. Conclusion: Osthole and psoralen could be absorbed into the bloodstream, and the absorption into bloodstream of psoralen could be promoted by Common Cnidium Fruit and Malaytea Scurfpea Fruit 1: 1 compatibility.