郁心效的抗抑郁作用及其机制研究

目的:确认郁心效的抗抑郁作用,并初步探讨其抗抑郁作用的机制.方法:小鼠随机分5组:模型组、阳性药物对照组、郁心效低、中、高剂量( 320,640,1 280 mg· kg -1)组,均ig7 d,于末次给药后建立5-羟色氨酸(5-HTP)诱导小鼠甩头和育亨宾小鼠致死增强模型,观察郁心效对5-HTP( 10 mg·kg-1,iv)诱导小鼠甩头行为和对小鼠皮下注射(sc)育亨宾(35 mg·kg-1)致死增强的影响；大鼠随机分6组:正常组、模型组、盐酸舍曲林(10 mg·kg-1)组、郁心效低、中、高剂量(220,440,880mg·kg-1)组,建立慢性不可预知性温和应激模型(CUMS),造模同时ig给药8周,观察郁心效对大鼠糖水偏嗜度、旷场行为和海马中5-羟色胺(5-HT),多巴胺(DA),去甲肾上腺素(NE)含量的影响.结果:郁心效可显著增加5-HTP诱导的小鼠甩头次数,对育亨宾诱导的小鼠致死量没有显著影响；可显著提高CUMS大鼠的糖水偏嗜度、旷场行为总评分和海马中5-HT,DA的含量.结论:郁心效具有明确的抗抑郁作用,其作用机制可能与5-HT系统有关.

Preliminary Study on Antidepressant Effect of Yuxinxiao and its Mechanism

Objective: To study the antidepressant effect and mechanism of Yuxinxiao. Method: Mice were randomly divided into five groups as follows:the model group,positive control group,Yuxinxiao groups(320,640,1 280 mg·kg^-1),all groups were administered ig for 7 days, 5-hydroxytryptophan(5-HTP)-induced head-twitch and yohimbine-induced lethality tests were used after the last administration to observe the impact of 5-HTP(10 mg·kg^-1,iv) induced head twitch behavior and yohimbine-induced(35 mg·kg^-1,sc) lethality in mice; rats were randomly divided into control group, model group, sertraline hydrochloride group(10 mg·kg^-1),Yuxinxiao groups(220,440,880 mg·kg^-1).The chronic unpredictable mild stress model was established by continuous gavage 8 weeks, the sucrose preference degree,open-field behavior and the content of 5-HT,dopamine(DA) and norepinephrine(NE) in hippocampus were tested to evaluate the empact of Yuxinxiao on CUMS models. Result: Yuxinxiao(320,640,1 280 mg·kg^-1,ig)ignificantly increased the frequence of 5-HTP(10 mg·kg^-1,iv) induced head-twitch in mice after 7 days of continuous administrations while it did not enhance the yohimbine-induced(35 mg·kg^-1,sc)lethality in mice. The contents of 5-HT and DA were increased remarkably in hippocampus of the model group treated with high dose of Yuxinxiao(880 mg·kg^-1) after 8 weeks of continuous administrations compared with model groups. Conclusion: Yuxinxiao has a clear antidepressant effect with manner of dose-dependent. The mechanism may be related to the system of 5-HT in central nervous system.