柔毛淫羊藿对去卵巢小鼠骨密度、破骨细胞和IL-1β的影响

目的:探讨单味中药柔毛淫羊藿( EPM)对去卵巢小鼠骨密度、破骨细胞和白介素-1β(IL-1β)的影响.方法:切除雌性小鼠双侧卵巢制备骨质疏松动物模型,随机分为模型组、己烯雌酚( DES,2 mg·kg-1)组及EPM水煎浸膏高、中、低剂量( 200,100,50 mg·kg-1)组,另设正常对照组.术后45 d开始给予相应药物,1次/d,60d后检测小鼠骨组织骨密度、破骨细胞数和骨组织中IL-1β.结果:模型组动物呈现明显的骨质疏松表现；给药组动物骨密度增加；EPM高、中、低剂量骨组织破骨细胞数分别为(1.9±1.0),(0.9±0.6),(0.8±0.4)个,明显低于模型组(3.4±1.6)个,(P ＜0.05或P＜0.01);EPM高、中、低剂量骨组织IL-1β阳性细胞数分别为(11.6±2.1),(12.4±2.2),(16.4±2.7)个,明显低于模型组(20.6±3.2)个(P＜0.01).结论:EPM能增加去卵巢骨质疏松症小鼠的骨密度、降低破骨细胞数和IL-1β的表达.

Effects of Epimedium pubescens Maxim. on Osteoporosis in Ovariectomized Mouse

Objective:To investigate the effect of Epimedium pubescens Maxim.(EPM)on osteoporosis in ovariectomized mouse.Method:Kunming mice were used in this study.Osteoposis model was estabished by ectomizing the ovarians of the mice.The mice were divided into six groups randomly:control group,model group,diethylstilbestrol positive control group,high dose of EPM group(200 mg·kg-1),middle dose of EPM group(100 mg·kg-1) and low dose of EPM group(50 mg·kg-1).Sixty days after administration,bone density,the osteoclast and intelukin(IL-1β) of the bone tissue were measured.Result:The model group animals had obvious osteoporosis.EMP increased bone density,and decreased the osteoclast number and IL-1β obviously.Conlusion:EPM can increase the bone density.EPM has an apparent role of anti-osteoporosis in ovariectomized mice.And it’s mechanism may be related to the reducing of the level of IL-1β.