小半夏加茯苓汤及其拆方对家兔离体胃肠运动的影响

目的:研究小半夏加茯苓汤(Xiaobanxia Fuling decoction,XBFD)及其拆方水提物对家兔离体胃肠运动的影响.方法:用生物信号分析系统记录小半夏加茯苓汤及其拆方提取物对家兔正常离体胃、肠平滑肌和工具药乙酰胆碱、阿托品所致离体胃、肠平滑肌收缩的影响,采集胃、肠平滑肌收缩的曲线和数据,比较用药前后胃、肠平滑肌收缩的振幅、频率和张力变化.结果:小半夏加茯苓汤及其拆方提取物对正常离体胃底和胃窦平滑肌自主活动具有不同程度的兴奋作用,对家兔正常离体十二指肠平滑肌的正常舒缩活动有抑制作用,加药前后比较显著差异(P＜0.01)；能拮抗氯化乙酰胆碱引起的胃肠平滑肌强直痉挛(P＜0.01)；对阿托品引起的十二指肠平滑肌舒缩活动减弱具有协同作用(P＜0.05),而对阿托品作用后的胃底和胃窦平滑肌有兴奋作用(P＜0.01),原方及拆方的作用强度表现为:小半夏加茯苓汤＞小半夏汤＞生姜＞半夏.结论:小半夏加茯苓汤及其拆方提取物对家兔离体胃肠运动具有双向调节作用,复方药效优于拆方.其作用途径不仅与M胆碱受体有关,还可能与其他调节因素有关.

Effect of the Xiaobanxia Fuling Decoction and Each Decomposed Group on Animal Gastroin Testinal Activity

Objective:To investigate the effect of Xiaobanxia Fuling decoction and each decomposed group on the isolated gastric and duodenal smooth muscle movement of rabbit.Method:The experiment uses the organs out of body,and the biologic function analyzer to record the effect of Xiaobanxia Fuling decoction and the aqueous extract of each decomposed group on the rabbits’ normal isolated gastric and duodenal smooth muscle and the contraction of isolated gastric and duodenal smooth muscle induced by Ach and atropine.The plot are collected,and the amplitude of vibration,frequency and intension of the isolated gastric and duodenal smooth muscle contraction before and after medication are compared.Result:The effect of Xiaobanxia Fuling decoction and the aqueous extract of each decomposed group on the the isolated gastric and duodenal smooth muscle movement can be recapitulated as the stomach contracts and the duodenum diastoles.It not only has effect on the normal isolated stomach and duodenum,but also has significant antagonistic effect on the rigidity and spasm of gastric and duodenal smooth muscle caused by acecoline(P<0.01);it has synergistic effect on the attenuation of the contraction of duodenal smooth muscle caused by atropine(P<0.05);and it has the effect of excitation on the smooth muscle of the fundus gastricus and the gastric antrum after the use of atropine(P<0.01).The drug potency of Xiaobanxia Fuling decoction and each decomposed group can be listed as:Xiaobanxia Fuling decoction>Xiaobanxia decoction>Rhizoma Zingiberis Recens>Rhizoma Pinelliae.Conclusion:Xiaobanxia Fuling decoction and the aqueous extract of each decomposed group has dual-effect on the isolated gastric and duodenal smooth muscle movement of rabbit;Xiaobanxia Fuling decoction is better than the decomposed group.The way that it plays the role has relationship not only with M choline receptor,but also with other regulatory factors.