大黄素联合吉西他滨对体外人胰腺癌细胞株BxPC-3生长及凋亡的影响

目的 探讨大黄素(emodin)联合吉西他滨（gemcitabine）对人胰腺癌细胞株BxPC-3的影响。 方法 人胰腺癌细胞株BxPC-3经不同浓度大黄素(0、10、 20、40、80、160 μmol/L)分别作用 24、48、72 h；及大黄素(40 μmol/L) 联合吉西他滨(20 μmol/L)作用72 h。应用Cell Counting Kit-8(CCK-8)法检测大黄素及大黄素联合吉西他滨对BxPC-3细胞的增殖抑制作用；流式细胞术检测BxPC-3细胞凋亡情况。 结果 大黄素对BxPC-3细胞的增殖抑制作用呈明显浓度和时间依赖性，40 μmol/L大黄素作用于BxPC-3细胞24、48、72 h后的细胞存活率分别为79.39%、46.35%、45.44%；大黄素(40 μmol/L) 联合协同吉西他滨(20 μmol/L)作用72 h后胰腺癌BxPC-3细胞的存活率仅为26.62%，与吉西他滨组（42.78 %）及大黄素组（47.18%)比较，差异有统计学意义（P<0.05）。大黄素(40 μmol/L)和吉西他滨(20 μmol/L)单独作用于BxPC-3细胞24 h后，诱导胰腺癌细胞早期凋亡率分别为（4.70±1.54）%和（11.20±1.41）%，与联合组［细胞凋亡率为（20.60±3.23）%］比较，差异均有统计学意义（P<0.05）。 结论 大黄素可显著抑制人胰腺癌BxPC-3细胞生长；大黄素有效增强吉西他滨对人胰腺癌BxPC-3细胞的增殖抑制作用；其协同作用以诱导胰腺癌细胞凋亡方式为主。

Effect of Emodin Combined Gemcitabine on the Growth and Apoptosis of Pancreatic Cancer Cell Line BxPC-3 in vitro

Objective To explore the effect of emodin combined gemcitabine（EG） on human pancreatic cancer cell line BxPC-3 in vitro.Methods BxPC-3 cells were treated with emodin alone in different concentrations（0,10,20,40,80,and 160 μmol/L,respectively） for 24,48,and 72 h,and EG（emodin 40 μmol/L＋ gemcitabine 20 μmol/L） for 72 h.The inhibition on BxPC-3 cell proliferation was detected by Cell Counting Kit-8 assay and the cell apoptosis of BxPC-3 was determined using flow cytometry.Results Emodin obviously suppressed the proliferation of BxPC-3 cells in a dose-and time-dependent manner.The survival rates of BxPC-3 cells by 40 μmol/L emodin for 24,48,and 72 h were 79.39%,46.35%,and 45.44%,respectively,while the survival rate of BxPC-3 cells acted by 72-h EG was only 26.62%,showing significant difference from that by gemecitabine alone（42.78%） and the emodin alone（47.18%）.The early apoptotic ratio of BxPC-3 cells induced by 24 h emodin（40 μmol/L） and gemecitabine（20 μmol/L） were 4.70%±1.54% and 11.20%±1.41% respectively,while early apoptotic ratio of BxPC-3 cells induced by EG was 20.60%±3.23%,showing significant difference from that induced by emodin or gemecitabine alone（P0.05）.Conclusions Emodin could significantly inhibit BxPC-3 cell growth.It could act synergistically with gemcitabine to inhibit the tumor proliferation of BxPC-3 cells.Its syndergistic action was achieved mainly through inducing pancreatic cancer cell apoptosis.