| Drp-1在糖尿病大鼠坐骨神经病变中的作用及白藜芦醇的干预影响 |
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| 引用本文： | 陆灵美,戴敏超,赵瑛,刘志民,邹俊杰.Drp-1在糖尿病大鼠坐骨神经病变中的作用及白藜芦醇的干预影响[J].标记免疫分析与临床,2010,17(4):250-255. |
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| 作者姓名： | 陆灵美 戴敏超 赵瑛 刘志民 邹俊杰 |
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| 作者单位： | 第二军医大学长征医院,神经内科,上海,200004;第二军医大学长征医院,内分泌科,上海,200003 |
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| 摘 要： | 探讨发动相关蛋白1（dynamin-related protein 1,Drp-1）在糖尿病大鼠坐骨神经中的作用及白藜芦醇干预的影响。采用链脲佐菌素（STZ）一次性腹腔注射诱导建立糖尿病大鼠模型;将制模成功的大鼠随机分为糖尿病对照组（DM组）、白藜芦醇低剂量治疗组（L治疗组）和白藜芦醇高剂量治疗组（H治疗组）,另设正常组（N组）。应用Real-time PCR检测各组大鼠坐骨神经中Drp-1的表达水平,免疫组化检测各组Bax、Bcl-2、Caspase-3的蛋白表达,电镜下观察各组坐骨神经的超微结构。结果表明,超微结构显示DM组大鼠坐骨神经髓鞘疏松、板层排列紊乱、线粒体肿胀变性,出现溶解破碎;白藜芦醇治疗组上述病变程度较轻。DM组与N组相比,Drp-1、Bax、Caspase-3蛋白表达明显升高,Bcl-2蛋白表达明显下降;治疗组与DM组相比,Drp-1、Bax、Caspase-3蛋白表达明显下降,Bcl-2蛋白表达明显升高。Drp-1蛋白表达升高促进糖尿病大鼠细胞凋亡,坐骨神经变性;白藜芦醇能降低Drp-1表达,减少细胞凋亡,对糖尿病大鼠坐骨神经起一定的保护作用,且疗效跟剂量相关。
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| 关 键 词： | 糖尿病大鼠 坐骨神经 发动相关蛋白1 白藜芦醇 凋亡 |
Roles of Drp-1 in Diabetic Rat Sciatic Nerve and Effects of Resveratrol Intervention on it |
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| LU Ling-mei,DAI Min-chao,ZHAO Ying,LIU Zhi-min,ZOU Jun-jie.Roles of Drp-1 in Diabetic Rat Sciatic Nerve and Effects of Resveratrol Intervention on it[J].Labeled Immunoassays and Clinical Medicine,2010,17(4):250-255. |
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| Authors: | LU Ling-mei DAI Min-chao ZHAO Ying LIU Zhi-min ZOU Jun-jie |
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| Institution: | LU Ling-mei,DAI Min-chao,ZHAO Ying,LIU Zhi-min,ZOU Jun-jie(Department of Neurology,Changzheng Hospital of the Second Military Medical University,Shanghai 200003,China) |
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| Abstract: | To explore the expression of Dynamin-related protein 1(Drp-1)in diabetic rat sciatic nerve and effects of resveratrol on its expression,models of diabetic rats were induced by a single intra-peritoneal injection of streptozotocin(STZ).The diabetic rats were randomly divided into the control group(DM group),low dose of resveratrol treated group(L treated group) and high dose of resveratrol treated group(H treated group),and normal group(N group) was also set up.The Drp-1 expression of each group was evaluate... |
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| Keywords: | Diabetic rat Sciatic nerve Dynamin-related protein 1 Resveratrol Apoptosis |
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