BALB/c小鼠渍疡性结肠炎远段结肠Cajal间质细胞分布变化

目的：建立BALB／c小鼠急、慢性溃疡性结肠炎（UC）动物模型，观察UC小鼠远段结肠组织中Cajal间质细胞（ICC）分布的变化，探讨ICC在UC肠功能紊乱发病中的作用。方法：将30只BALB／c小鼠随机分为健康对照组、急性和慢性UC组，每组各10只。通过饮用50g／L葡聚糖硫酸钠（DSS）方法建立BALB／c小鼠急、慢性UC动物模型，对照组仅饮用蒸馏水。应用免疫荧光、激光共聚焦荧光定量等技术检测ICC在UC小鼠远段结肠组织内分布的变化。结果：1．健康对照组远段结肠组织ICC主要分布在黏膜下丛（IC—SM）和肌间丛（IC—MY），IC—MY在切面上几乎呈现连续性分布，相互连接形成网络状结构；2．急性UC组远段结肠组织内ICC的分布较健康对照和慢性UC组明显减少（Pa〈0、001），IC—MY的网络状结构完全破坏，残存ICC形态也出现异常；3．慢性UC组远段结肠组织ICC的分布较健康对照组减少（P〈0．05），但较急性UC组显著增加（P〈0．001），其形态接近正常，但IC—MY的连续性分布不明显，未能形成正常的网络状结构。结论：UC小鼠远段结肠组织内ICC分布减少，形态异常，推测与UC小鼠肠功能紊乱有一定相关性。

Distribution of Interstitial Cells of Cajal in Distal Colon in BALB/c Mice with Ulcerative Colitis

Objective To establish acute and chronic ulcerative colitis model in BALB/c mice and explore the relationship between the changes of interstitial cells of Cajal in distal colon and gastrointestinal dysfunction associated with ulcerative colitis.Methods Thirty BALB/c mice were randomly divided into three groups.The experimental groups were fed with 5% dextran sulfate sodium(DSS)solution for 7 days and 5% DSS solution for 7 days followed by distilled water for 14 days to induce acute and chronic ulcerative colitis,respectively.The distribution of interstitial cells of Cajal in distal colon were detected by immunofluorescence and laser confocal scanning microscope.Results 1.In control group,interstitial cells of Cajal were mainly distributed in submucosal plexus(IC-SM)and myenteric plexus(IC-MY)of the distal colon,and also distributed in circular muscle layer and longitudinal muscle layer(IC-IM).IC-MY were distributed continuously between the circular and longitudinal muscle layers and formed a network.2.Compared with control group and chronic UC group,interstitial cells of Cajal decreased obviously in the distal colon of acute group(Pa<0.001).The IC-MY network were destroyed and the configuration of the residual ICC were abnormal.3.In chronic group,ICC were less than those of control group(P<0.05),but were more than those of acute group(P<0.001).Their configuration were close to normal,but the IC-MY did not form a normal network.Conclusion Decreased expression of interstitial cells of Cajal in the distal colon may play an important role in the gastrointestinal dysfunction associated with ulcerative colitis.