非HLA因素致大鼠肾移植CAN加快模型的建立

目的建立大鼠异体肾移植慢性移植肾肾病(CAN)加快模型,为研究非HLA免疫因素及非免疫因素致CAN的病因、病理及病理生理机制提供平台。方法采用雄性Fisher大鼠和Lewis大鼠分别作为供-受体,并以假手术组作为对照,进行异体原位肾移植。受体移植术前对供肾进行强化冷缺血处理1 h,于术后4 w,8 w,12 w分别观察各受体血肌酐和移植肾组织病理变化情况。结果移植术后4周Fisher-Lewis组开始出现血肌酐的升高及移植肾CAN的病理改变,8~12周病变渐趋明显,与对照组比较有统计学差异(P<0.05)。结论以Fisher和Lewis近交系大鼠作为供-受体建立的大鼠异体肾移植CAN加快模型是一种可靠、实用和研究价值高的肾移植实验动物模型。

Establishment of a Model of Chronic Allograft Nephropathy in Rat Caused by Non-HLA Factors

Objective To set up a chronic allograft nephropathy(CAN) model in rat for the studying in chronic rejection caused by non-HLA and non-immunologic factors.Methods We used Fisher rats as donors and Lewis rats as recipients with modified Kamada's surgical procedures.Before recipient's operation,the donor kidney was performed with intensive ischemia/reperfusion injury.The recipients were given cyclosporine after operation to prevent acute rejection.Results In the 4~th,8~th and 12~th weeks after transplantation,the recipients' serum creatinine levels significantly increased than the control group(P<0.05 or P<0.001).And the transplanted kidneys gradually showed obviously pathological characteristics of CAN during the period.Conclusion This model is a reliable,valued and useful platform in studying chronic allograft nephropathy of renal transplantation.