急性白血病zo-1基因启动子区甲基化状态研究

本研究探讨健康人和急性白血病(AL)不同时期患者zo-1基因启动子区甲基化状态差异。采用硫化测序特异性PCR(BS-PCR)方法对白血病细胞系、AL患者及健康人骨髓标本进行zo-1基因启动子区甲基化状况检测。结果显示:在健康人骨髓中呈低甲基化状态(1.9%),初治、复发和完全缓解的AL患者骨髓中zo-1基因呈高甲基化状态,甲基化率分别为93.2%、66.9%及16.4%,明显高于健康人,差异均有统计学意义(p0.05)。结论:与健康人相比,急性白血病患者骨髓细胞zo-1基因呈高甲基化状态,且AL不同时期的患者中zo-1基因都发生了不同程度的甲基化改变,对zo-1基因甲基化状态的分析有助于监测AL患者疾病的进展。

Methylation Status of zo-1 Gene Promoter in Acute Leukemia

This study was purposed to investigate the difference of zo-1 gene promoter methylation between healthy individuals and acute leukemia patients. BS-PCR method was used to detect the status of zo-1 gene methylation in healthy individuals, acute leukemia patients and leukemic cell line NB4 cells. The results showed that zo-1 gene was hypomethylated in bone marrow samples from healthy individuals（ 1.9% ）. In newly diagnosed AL and relapsed patients, the rate of zo-1 gene methylation was 93.2% and 66.9% respectively, while it was 16.4% in AL patients in complete remission, which was much higher than that in healthy individuals. There was significant difference between them. It is concluded that as compared with healthy individuals, zo-1 gene in acute leukemia patients is hypermethylated and with different degrees in various phases of leukemia. Analysis of zo-1 gene methylation status may be useful to monitor the development of acute leukemia.