Procoagulant Activity of Human Neuroblastoma Cell Lines, in Relation to Cell Growth, Differentiation and Cytogenetic Abnormalities |
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| Authors: | Noriko Esumi Shinsaku Imashuku Kentaro Tsunamoto Shinjiro Todo Shinichi Misawa Toshihiro Goto Yoshio Fujisawa |
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| Affiliation: | Department of Pediatrics, Kyoto Prefectural University of Medicine, Kawaramachi, Kamigyoku, Kyoto 602;Children's Research Hospital, Kyoto Prefectural University of Medicine, Kawaramachi, Kamigyoku, Kyoto 602;Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kawaramachi, Kamigyoku, Kyoto 602;Shionogi Bio-medical Laboratory, Mishima, Settsu, Osaka 618 |
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| Abstract: | Procoagulant activity (PCA) was investigated in relation to cell growth, differentiation, and cytogenetics in seven human neuroblastoma cell lines. Before 5-bromodeoxyuridine (BrdUrd) treatment, PCA was notably heterogeneous, with the highest activity in NCG (S-type in morphology) 40-to 100-fold greater than the lowest activity in SK-N-D2 (N-type). PCA was not related to 1p abnormalities. After BrdUrd treatment at 5 μg/ml for 6 days, PCA increased 6.8-fold in GOTO and 2.7-fold in SK-N-DZ with associated growth inhibition and morphological changes (I-type morphology converted to S-type in GOTO and N-type converted to an advanced N-type in SK-N-DZ). In contrast, only growth suppression was observed in 2 other cell lines, and no changes in PCA, growth or morphology were induced in the remaining 3 cell lines. |
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| Keywords: | Neuroblastoma Procoagulant activity Chromosome abnormality 5-Bromodeoxyuridine |
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