用鼠疫EV菌对草原雕进行模拟感染的实验报告

1984年4月～1987年4月我们用鼠疫EV菌感染的小白鼠饲喂草原雕(Aquila rapax nipa-lensis)进行了鼠疫模拟感染试验。结果,在收集的吐物中进行鼠疫FI抗原及鼠疫菌检测均为阴性。从受试的草原雕血清中也未能检出FI抗体。用鼠疫EV菌经草原雕皮下注射感染,在其血清中能检出FI抗体,且至少可保持240天。用不同N浓度的HCI及NaOH溶液水解鼠疫EV菌,对用鼠疫EV菌感染的小白鼠在2N以上的HCl及0.4N以上的NaOH溶液中作用12小时,可使小白鼠全部或部分水解,用抗体中和、反向血凝以及细菌学方法对上述溶液进行检测,在1N以上的HCl与0.4N以上的NaOH水解液中未能检测到FI抗原,细菌培养阴性。而在1N以下的HCl与0.4N NaOH以下的水解液中,则能检测到FI抗原,并培养出鼠疫菌。在鼠疫EV菌菌体的水解液中,检出FI抗原及鼠疫菌的N浓度比前者稍低。实验结果证明足够N浓度的酸、碱溶液,作用于EV菌体或感染鼠,均能使菌体蛋白质分解,而丧失抗原性。

SIMULATED INFECTION IN AQUILA RAPAX NIPALENSIS WITH EV STRAIN OF YERSINIA PESTIS

Simulated infections were undertaken in the vulture Aquila rapax Mipalensis with EV strain of Yersinia pestis from April, 1984 to April, 1987. Neither FI-antigen and Y.pestis from its spit nor FI-antibody from its serum could be detected after the vulture had been fed with mice infected with EV strain. But FI-anti-body was detected from its serum when the animal had been subcutaneously inoculated with EV strain and, the positive detections lasted for more than 240 days. To explain the above mentioned results, hydrolysis of EV strain was made with HC1 and NaOH solutions of different normal concentrations. Results showed that infected mouse tissues could be decomposed by 2.0N HCI or 0.4N NaOH and above in 12 hours and, no Fl-antigen or bacteria could be detected from the hyttrolyzates by methods of antibody neutralization, reverse hemag-glutination and bacterial culture. But Fl-antigen and Y.Pestis could be detec-cted from the hydrolyzates when infected mouse tissues were reacted by HCI below 1.0N or NaOH below 0.4N.And EV strain of Y .pestis itself could be decomposed by lower concentrations of HCI or NaOH than those needed for mouse tissues. The experimental results indicated that cells of EV strain and infected mouse tissues could be decomposed by acidic or basic solutions above certain concentrations, thereby, lost their antigenicity.